Sequences from confirmation amplicon


Confirmation sequences for line 773B10 and hit At2g44350 are shown.
Line ID 773B10
Locus At2g44350 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:18320085
Primers used for PCR 60gp - 8474, show primer details
   
Sequence Name 55-K112131-0022-773-B10C-60gp
GenBank Accession LN508855 [GenBank]
Sequencing primer gene specific primer, 60gp: 5'-GAGAATTTGCCTTGAAGCATCTAC-3'
Sequence (A. th genome BLAST matches underlined) 
>55-K112131-0022-773-B10C-60gp
TTTTTTCTTTCCTTTTCTCTTTTCTGTTCGGTTCTCAAAATTCTATCAATGTTCAGTAGC
TAACAAAATCCTTCCAACTCTGTAGGTGTCAAAGCTTTATGAAGTTGTGCCTCCTGTTCT
CACTGAGCTTGGAAAGGTAGTATCTCTATCCTTCGTTTACCATGGTGATCCTTCAATATT
CCACCAACAACTCCACAACACCTTACCTACATGCTAGGGGCTTATGAATCTCTTGCGAAT
CAATTTTTTATTTACTTGCGAATCCGGGAAATCTACATGGATCAGCAATGAGTATGATGG
TCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATG
TCCGC
   
Sequence Name 39-K112130-0022-773-B10C-8409
GenBank Accession LN503398 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>39-K112130-0022-773-B10C-8409
TTGATTCGCAAGAGATTCATAAGCCCCTAGCATGTAGGTAAGGTGTTGTGGAGTTGTTGG
TGGAATATTGAAGGATCACCATGGTAAACGAAGGATAGAGATACTACCTTTCCAAGCTCA
GTGAGAACAGGAGGCACAACTTCATAAAGCTTTGACACCTACAGAGTTGGAAGGATTTTG
TTAGCTACTGAACATTGATAGAATTTTGAGAACCGAACAGAAAAGAGAAAAGAGAAAGAA
AAAAAGTACCAGCTGGAAAAGAGGGTCGTCAGGTAGATGCTTCAAGGCAAATTCT

Last Updated on 06.12.2018 16:11