Sequences from confirmation amplicon


Confirmation sequences for line 774D08 and hit At4g28770 are shown.
Line ID 774D08
Locus At4g28770 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:14213476
Primers used for PCR 23dr - 8474, show primer details
   
Sequence Name 78-K110174-0022-774-D08C-23dr
GenBank Accession LN513702 [GenBank]
Sequencing primer gene specific primer, 23dr: 5'-GGAAGTCTCTTCACAATGAGCTTT-3'
Sequence (A. th genome BLAST matches underlined) 
>78-K110174-0022-774-D08C-23dr
TTAGTTTGTTTGGCCTCATCAACTATATCAAAATTTTGAACTATTACATTAATGATAATG
AAAATTTTCGTTAATTAATAAATTTTCGTTAATGATTAATTAGTAAGCTTCGGAATATAT
CCTATCTGTCAAAACAACATTTTTCTGTTTTGTCCGAAATCATCAAAGACTCTTCCTTCG
GCGATCGCTGCTCAGAAGTTTGATTTCGATATCAAGAATCGTGATAGAAAAAATGCGACA
CAATTGTTGCCATGTCTCGTTCGCTTCGATTCTCAGGATATATTCAATTGTAAATGGCTT
CATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGA
AAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGC
   
Sequence Name 68-K110173-0022-774-D08C-8409
GenBank Accession LN512037 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>68-K110173-0022-774-D08C-8409
ATCGGAATGAGCCTTTACAATTGAATATATCCTGAGAATCGAAGCGAACGAGACATGGCA
ACAATTGTGTCGCATTTTTTCTATCACGATTCTTGATATCGAAATCAAACTTCTGAGCAG
CGATCGCCGAAGGAAGAGTCTTTGATGATTTCGGACAAAACAGAAAAATGTTGTTTTGAC
AGATAGGATATATTCCGAAGCTTACTAATTAATCATTAACGAAAATTTATTAATTAACGA
AAATTTTCATTATCATTAATGTAATAGTTCAAAATTTTGATATAGTTGATGAGGCCAAAC
AAACTAAATGGGCTAGAATTCGAATAAATGGGCTTAGGGTCAGGAAAGCTCATTGTGAAG
AGACTTC

Last Updated on 06.12.2018 16:11