Sequences from confirmation amplicon


Confirmation sequences for line 775A11 and hit At3g52990 are shown.
Line ID 775A11
Locus At3g52990 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:19649631
Primers used for PCR CS40 - 8474, show primer details
   
Sequence Name 38-K105920-0022-775-A11C-CS40
GenBank Accession LN502983 [GenBank]
Sequencing primer gene specific primer, CS40: 5'-GTTGTTGTTGTTGTGAGCAACTTT-3'
Sequence (A. th genome BLAST matches underlined) 
>38-K105920-0022-775-A11C-CS40
TTGAACGGTGTTTGTTATTCGGAGAAGAAGAAGAAGAAGAACTGAAGAAGCATATTCGAC
GGTCACCACCAAACCTCTTCCGTCGGATTTTCACTCTCCGTTTATCGAACATCTGAGATC
TCTCTCCACTTCCGTCACCGAAGATGCATTCCAGTCATCTTCTTCTCGAGGAGCCGATCA
GGATGGCTTCAATCCTCGAGCCTTCCAAATCTGTGAGCTCTCTCTCTCTCTCTCTCAGCT
TGATAATTTAGTATCCCAGTTCTTCATCGATTTGCAATTTCGCATGGATTCGATTTTGTT
GTTGAGGTTGTTTCTCTGATCTCTTTTTGATGTATTTTCTCAGAGTTTCTTCCCGGCATT
GACTAAGATCGTCGGAACTCTGGGTCCTAAATCCCGATCCGTCGAGGCTCTCTCCGGCTG
TCTCAAAGCCGGCGTGTCTGGTACTAACGATTTACTTCTCTTTTTTTTTGGGGGTTTGCT
AACTTGATTTGTGTTCGGTCTCACTGATAACTGTTCATGTTCTGTCATTTAGGGGCTCGA
TTTGATTTCTCGGGGGGAGATGCTGATTATCCCCGGGAGACACTTGATAATTTGAAAGTT
GCTGTGAGGAGCACTAAAAAGCTTTGGGCTGAAGGTTTTCTCTCCTCTAATTTTTTTAAC
CGGAGGGATCTTTTTTTTAGGGGCTATATGTATTTGATTGGAGATTTCTTCGGCGTTTGA
TCGGGTTATCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACGTGGAT
CAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCATTTTTTT
   
Sequence Name 38-K105921-0022-775-A11C-8409
GenBank Accession LN502984 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>38-K105921-0022-775-A11C-8409
CGGACTGAGCCATTTACAATTGAATATATCCTGATAACCTGATCAAACGCAGAAGAAATC
TCAAATCAAATACATATAGCACATAAAAAAAAGATCCATCAGATTAAAAAAATAAGAGAA
GAGAAAACATACAGCACAAAGCTTCTTAGTGCTCCTCACAGCAACTTTCAAATTATCAAG
TGTCTCCTGGTGATAATCAGCATCTCCCCACGAGAAATCAAATCGAGCCACTAAATGACA
GAACATGAACAGTTATCAGTGAGACCGAACACAAATCAAGTTAGCAAACCCCAAAAAAAA
AAAAAAAGTAAATCGTTAGTACCAAACATGCCGGCTTTGAAACAGCCGGAAAGAGCCTCG
ACGGATCGGGATTTAGGACCTAAAGTTCCAACAATCTTAGTCAATGCCGGGAAAAAACTC
TGAGAAAATACTTCAAAAAGAGATCAAAGAAACACCCTCAACAACAAAATCGAATCCTTG
CGAAATTGCAAATCGATAAAAAACTGGGATACTAAATTATCAAGCTAAAAAAGAGAGAGA
GAGACCTCACAAATTTGGAAGGCTCGAGGATTGAACCCATCCTGATCGGCTCCTCGAAAA
AAAAATGACTGGAATGCATCTCCGGTGACGGAAGTGGAGAGAAATCTCAAATGTTCGATA
AACGGAGAGTGAAAATCCAACGGAAGAGGTTTGGTGGTGACCGTCAAATATGCTTCTNCA
GTTCTTCTTCTTCTTCTTCNCCAAATAACAAACACCGTTCAATAATATCTCACGCAGCGG
AAGACAAATAAATAAATAAAGGTTGCTCCA

Last Updated on 06.12.2018 16:11