Sequences from confirmation amplicon


Confirmation sequences for line 776F07 and hit At1g53060 are shown.
Line ID 776F07
Locus At1g53060 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:19776436
Primers used for PCR 28oh - 8474, show primer details
   
Sequence Name 41-K114782-0022-776-F07C-28oh
GenBank Accession LN504181 [GenBank]
Sequencing primer gene specific primer, 28oh: 5'-AGTTTCGACTTCATTTACTTCGGA-3'
Sequence (A. th genome BLAST matches underlined) 
>41-K114782-0022-776-F07C-28oh
GTATGAGCAAGTCAGGAGCAATTGGATTGAGCCGTGACAATGTCCCATTCTCTCATGGTC
GAGCCATTTTCATCAATCCAGTCCCTTTTAAGCCTAGTTCTACTTCTTCCTCTGTCTACT
CTTTCAAAACTTCTTTCTATTTCGTCATTAGTCCTCGCCCGAAAAATCCTAATCCCGGTC
ATGGCCTCGCCTTTATCATCGTCCCCAACGATAGAAACGACTCCGCTTCTGGTTTAGGCT
ATCTCAGTCTTGTGAACCGGTTTAGCAACGGTAATCCCAAGAACCATCTCTTTGCGGTTG
AGTTTGATGTTTTTAAAGACAAGTCTCTTGGCGACATTAACGGATATATTCAATTGTAAA
TGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGA
AAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCANAAATGTAGATATCCGCA
   
Sequence Name 41-K114769-0022-776-F07C-8409
GenBank Accession LN504180 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>41-K114769-0022-776-F07C-8409
CCGGGATTAGGAATTTTCGGGCGAGGACTAATGACGAAATAGAAAGAAGTTTTTGAAAGA
GTAGACAGAGGAAGAAGTAGAACTAGGCTTAAAAGGGACTGGATTGATGAAAATGGCTCG
ACCATGAGAGAATGGGACATTGTCACGGCTCAATCCAATTGCTCCTGACTTGCTCATACC
ATCAGGCTCTGAAGCGTACTCGGCGTCTCCATGGAATGTCATCAGATCAGTTCCATTTCC
GAA

Last Updated on 06.12.2018 16:11