Sequences from confirmation amplicon


Confirmation sequences for line 777A03 and hit At2g37900 are shown.
Line ID 777A03
Locus At2g37900 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:15864476
Primers used for PCR CS42 - 8474, show primer details
   
Sequence Name 25-K107428-0022-777-A03C-CS42
GenBank Accession LN497471 [GenBank]
Sequencing primer gene specific primer, CS42: 5'-CGTTTTTCATCAAACAAGCAATAA-3'
Sequence (A. th genome BLAST matches underlined) 
>25-K107428-0022-777-A03C-CS42
TTCCTCCGCTTCATGTTTACACTTACCGCCCTCACATTGATCATCTCACTCTATGTCTAT
GAGAAACTCCTCGTTCCCTTGCTAAGAAGCATCACACGGAATCAAAGAGGCATCAACATT
CTCCAAAGAATCGGGACCGGTATGATTTTCTCCCTCATCACCATGATCATTGCAGCTCTA
GTTGAGAAACAACGATTGGACCGTACCAACAACAACAAACCCATGAGTGTGATATGGCTA
GCTCCTCAGTTCATGGTCATTGGCTTTGCGGACGCTTTCACACTAGTTGGACTCCAAGAG
TACTTTTACCACCAAGTCCCTGACTCCATGAGAAGCTTAGGTATAGCGTTTTACCTAAGC
GTGATAGGTGCGGCTAGCTTCCTTAACAATCTCCTGATCACAGCCGTTGATACTCTCGCG
GAGAATTTCTCGGGGAAGAGTTGGTTCGGAAAGGACCTAAACAGCAGCCGCTTGGACCGG
TTCTACTGGTTTCTTGCTGGTGTTATCGTATATTCAATTGTAAATGGCTTCATGTCCGGG
AAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATT
ACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCACGGTTATT
   
Sequence Name 09-K107427-0022-777-A03C-8409
GenBank Accession LN490206 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>09-K107427-0022-777-A03C-8409
ATTCGGAATGAGCCATTTACAATTGAATATACGATAACACCAGCAAGAAACCAGTAGAAC
CGGTCCAAGCGGCTGCTGTTTAGGTCCTTTCCGAACCAACTCTTCCCCGAGAAATTCTCC
GCGAGAGTATCAACGGCTGTGATCAGGAGATTGTTAAGGAAGCTAGCCGCACCTATCACG
CTTAGGTAAAACGCTATACCTAAGCTTCTCATGGAGTCAGGGACTTGGTGGTAAAAGTAC
TCTTGGAGTCCAACTAGTGTGAAAGCGTCCGCAAAGCCAATGACCATGAACTGAGGAGCT
AGCCATATCACACTCATGGGTTTGTTGTTGTTGGTACGGTCCAATCGTTGTTTCTCAACT
AGAGCTGCAATGATCATGGTGATGAGGGAGAAAATCATACCGGTCCCGATTCTTTGGAGA
ATGTTGATGCCTCTTTGATTCCGTGTGATGCTTCTTAGCAAGGGAACGAGGAGTTTCTCA
TAGACAGTGAGTGAGATGATCAATGTGAGGGCGGTAAGTGTAAACATGGAAGCTGGAGGA
ACTGTGAAGCCACCGATGTGTCTGTCCATGGTTATTGCTTGTTTGATGAAAAACG

Last Updated on 06.12.2018 16:11