Sequences from confirmation amplicon


Confirmation sequences for line 777C04 and hit At1g80090 are shown.
Line ID 777C04
Locus At1g80090 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:30131852
Primers used for PCR 35dl - 8474, show primer details
   
Sequence Name 24-K109936-0022-777-C04C-35dl
GenBank Accession LN497034 [GenBank]
Sequencing primer gene specific primer, 35dl: 5'-AACACTGTTTTAGCCCTCACTAGC-3'
Sequence (A. th genome BLAST matches underlined) 
>24-K109936-0022-777-C04C-35dl
TCTTAATCCTAAGTTAACGCTTGTAATGTTTAATACCAGGCTAGAGTGAAAGATGCAATA
CAATGCATGAGTATAGCAATGCTCAATGCGGTTCCTATCGTCGAAGCCTCTGGTGAGGGA
GAGGATCATAAACAGCTCGTCGACGTAGGTGTCTTTACTTATACATGTATCTATCAGTAT
CTATTAAGATCAAATGTTGATTAATTTGGTGTTTGCTGGTGCAGGGGAAAAATCGGAGAG
TGGTGGGGACATTCTCGGCTAGTGATCTGAAGGGATGTCATTTAGCCACATTGCGTTCGT
GGTTGCCGCTAAATGCACTAGAGTTCGTCGAGAAAATCCCAAGAACTCTCTTGTTCACCG
CTGCTACCTCAACACCAGGGAGGGAGCTCGTGACGTGTCACGTGACATCAACCTTGGCCC
AGGTGATCCATATGGTCACCACGAAACGTGTACACAGAGTTTGGGTCGTGGACCAGAACG
GTGGGCTACAAGGCCTTGTTTCTCTTACTGACATCATCGCTGTGGTCCGATCCGCTTTGC
TTTCCGGAGCTCCGGATCTGTTTTAAATTGTAAATGGCTTCATGTCCGGGAAATCCACCT
GGATCAGCAATGAGTATGATGGTCCATATGGAGAAAAAGAAAGAGTAATTTCCAATTTTT
TTTCAATTCAAAAATGTAGATG
   
Sequence Name 08-K109935-0022-777-C04C-8409
GenBank Accession LN489776 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>08-K109935-0022-777-C04C-8409
ATTTAAACAGATCCGGAGCTCCGGAAAGCAAAGCGGATCGGACCACAGCGATGATGTCAG
TAAGAGAAACAAGGCCTTGTAGCCCACCGTTCTGGTCCACGACCCAAACTCTGTGTACAC
GTTTCGTGGTGACCATATGGATCACCTGGGCCAAGGTTGATGTCACGTGACACGTCACGA
GCTCCCTCCCTGGTGTTGAGGTAGCAGCGGTGAACAAGAGAGTTCTTGGGATTTTCTCGA
CGAACTCTAGTGCATTTAGCGGCAACCACGAACGCAATGTGGCTAAATGACATCCCTTCA
GATCACTAGCCGAGAATGTCCCCACCACTCTCCGATTTTTCCCCTGCACCAGCAAACACC
AAATTAATCAACATTTGATCTTAATAGATACTGATAGATACATGTATAAGTAAAGACACC
TACGTCGACGAGCTGTTTATGATCCTCTCCCTCACCAGAGGCTTCGACGATAGGAACCGC
ATTGAGCATTGCTATACTCATGCATTGTATTGCATCTTTCACTCTAGCCTGGTATTAAAC
ATTACAAGCGTTTAACTTAGGATTTAATGAATAAAGATGAAAATTCTTGACTTGTGAAAT
TTACCTGGCTAGTGAGGGCTAAAACAGTGT

Last Updated on 06.12.2018 16:11