Sequences from confirmation amplicon


Confirmation sequences for line 777D11 and hit At5g10530 are shown.
Line ID 777D11
Locus At5g10530 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:3326823
Primers used for PCR EU63 - 8474, show primer details
   
Sequence Name 47-K103731-0022-777-D11C-EU63
GenBank Accession LN506180 [GenBank]
Sequencing primer gene specific primer, EU63: 5'-AAACCCAATTGTAACTTCTGATGG-3'
Sequence (A. th genome BLAST matches underlined) 
>47-K103731-0022-777-D11C-EU63
TGATGTAGATAGGCTTGAATTCTCCAGAGGATCAGATGTTAAGTCGTAAGTCCAAGAGAC
ACTCAAGTTTCTTCTAGCGGAATCATAGAATATCAGGACACGGCCTATATCTTGGTTGTG
TGACGTTGCATTCCAAGAAGTGTAGTTAGAAGAAACAAGAGAGTTGTTGTTGATTCCTAC
ATGGGATTTGACATCGAGAGGATCCCATTCTGGATTAGTAAATGTGTCGAACTCGACATA
AACAAGTGGGAAAGCAGAAGACTGATTATTGGTTCCATTGAATAGACCCAAGAAACCACC
AGCTGAGTTGGGAGGCAATTGGATTCTCGCTGGAGCTAGAAAGAAAGCAAACCCATGACC
GTAATTACCATACCCAACATTACGGGTATCAATTCTGAAGGAGAAACGCGTACTAAAATC
CGAAGGCTTACTGGTACCTGGATTCCATAAAGGAACCTGCTTACCATAAGTAGCCCAACC
GGCACGGCATGTGTAGTCAATGTTGGTAAGCTCAACAGCCCCATTTGCTCTTGCATCTCC
TTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAAT
ATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCANAAATGTAG
   
Sequence Name 63-K103732-0022-777-D11C-8409
GenBank Accession LN510973 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>63-K103732-0022-777-D11C-8409
ATCGGACATGAGCCATTTACAAGGAGATGCAAGAGCAAATGGGGCTGTTGAGCTTACCAA
CATTGACTACACATGCCGTGCCGGTTGGGCTACTTATGGTAAGCAGGTTCCTTTATGGAA
TCCAGGTACCAGTAAGCCTTCGGATTTTAGTACGCGTTTCTCCTTCAGAATTGATACCCG
TAATGTTGGGTATGGTAATTACGGTCATGGGTTTGCTTTCTTTCTAGCTCCAGCGAGAAT
CCAATTGCCTCCCAACTCAGCTGGTGGTTTCTTGGGTCTATTCAATGGAACCAATAATCA
GTCTTCTGCTTTCCCACTTGTTTATGTCGAGTTCGACACATTTACTAATCCAGAATGGGA
TCCTCTCGATGTCAAATCCCATGTAGGAATCAACAACAACTCTCTTGTTTCTTCTAACTA
CACTTCTTGGAATGCAACGTCACACAACCAAGATATAGGCCGTGTCCTGATATTCTATGA
TTCCGCTAGAAGAAACTTGAGTGTCTCTTGGACTTACGACTTAACATCTGATCCTCTGGA
GAATTCAAGCCTATCTTACATCATTGATCTCTCAAAGGTACTGCCATCAGAAGT
Primers used for PCR BB07 - 8474, show primer details
   
Sequence Name 55-K118446-0022-777-D11C-BB07
GenBank Accession LN508940 [GenBank]
Sequencing primer gene specific primer, BB07: 5'-AAAGTTTGACTCTGCTTGTTCACC-3'
Sequence (A. th genome BLAST matches underlined) 
>55-K118446-0022-777-D11C-BB07
GTCTTCCTTTGAAACTTGAGTTCCTTTGTGATTATCATAAGCTGTCTTACAATTTAGGAT
GAGTATCAAACATGTCTTCCTCATTTTTCTAATCTGTTATAAGCAATCAATACAGAGATA
TGATCTGTTTACTCTTAGGGTCAACTATATGGTGACTTTTATGGTTGTTTATCAAAAAAT
TTAATTCCTGTGATAGCTTGGATCACGCTCTCAAATGATTTCATTCTTATAATCAAGGTT
TAGTCATTGACTTGCTGATAAATATACATATCAGTTGCACCAATTTTGTCTCTTGAGCTA
ATTGTTGTTTCTGCCTGCAAATGGCCAACTCAATTCTGTTATTTTCCTTTGTTTTGGTTC
TCCCTTTTGTCTGTTCAGTTCAATTTAACATATCTCGTTTTGGATCAGATGTTTCTGAAA
TAGCATACCAAATGGCTTCATGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGC
AATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATT
CAAAAATGTAGATATCCGC
   
Sequence Name 15-K118445-0022-777-D11C-8409
GenBank Accession LN493183 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>15-K118445-0022-777-D11C-8409
CATTTCATGAAGCCATTTGGTATGCTATTTCAGAAACATCTGATCCAAAACGAGATATGT
TAAATTGAACTGAACAGACAAAAGGGAGAACCAAAACAAAGGAAAATAACAGAATTGAGT
TGGCCATTTGCAGGCAGAAACAACAATTAGCTCAAGAGACAAAATTGGTGCAACTGATAT
GTATATTTATCAGCAAGTCAATGACTAAACCTTGATTATAAGAATGAAATCATTTGAGAG
CGTGATCCAAGCTATCACAGGAATTAAATTTTTTGATAAACAACCATAAAAGTCACCATA
TAGTTGACCCTAAGAGTAAACAGATCATATCTCTGTATTGATTGCTTATAACAGATTAGA
AAAATGAGGAAGACATGTTTGATACTCATCCTAAATTGTAAGACAGCTTATGATAATCAC
AAAGGAACTCAAGTATATCAAAGAGAAGACTTATTTTTTCCTCAAGCCAATGGGTGAACA
AGCAAGTCCAAACTTT

Last Updated on 06.12.2018 16:11