Sequences from confirmation amplicon


Confirmation sequences for line 777H06 and hit At3g45430 are shown.
Line ID 777H06
Locus At3g45430 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:16661284
Primers used for PCR NF30 - 8474, show primer details
   
Sequence Name 04-K102429w-0022-777-H06C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>04-K102429w-0022-777-H06C-8409
CATTTACAATTGAATATATCACAACAGCAGCGGTTGGCACTGTAGGGTACATGGCACCTG
AGCTAATAACAATGGGAGCTTCCACTATAACCGATGTATATGCTTTCGGTGTTTTCTTGC
TTGAAGTAGCCTGTGGGAGGAAACCTGTGGAATTCGGGGTGCAAGTTGAGAAACGATTTC
TGATCAAATGGGTTTGTGAATGCTGGAAGAAAGATTCTTTGCTTGATGCTAAAGATCCAA
GATTGGGAGAAGAATTTGTACCCGAGGAAGTCGAGTTGGTTATGAAACTCGGGCTGCTAT
GTACAAACATTGTGCCAGAATCTAGACCAGCAATGGGACAAGTGGTTCTTTACTTAAGTG
GGAACTTACCATTGCCGGATTTCTCACCATATACACTTGGAATCGGCTCGTTTACTCCTG
TAGTTGTCGATGCAGCCTCACTTACCGTCTCATTTACTTCGAGG
   
Sequence Name 20-K102430-0022-777-H06C-NF30
GenBank Accession LN495159 [GenBank]
Sequencing primer gene specific primer, NF30: 5'-CCTCGAAGTAAATGAGACGGTAAG-3'
Sequence (A. th genome BLAST matches underlined) 
>20-K102430-0022-777-H06C-NF30
ACGAGCCGATTCCAAGTGTATATGGTGAGAAATCCGGCAATGGTAAGTTCCCACTTAAGT
AAAGAACCACTTGTCCCATTGCTGGTCTAGATTCTGGCACAATGTTTGTACATAGCAGCC
CGAGTTTCATAACCAACTCGACTTCCTCGGGTACAAATTCTTCTCCCAATCTTGGATCTT
TAGCATCAAGCAAAGAATCTTTCTTCCAGCATTCACAAACCCATTTGATCAGAAATCGTT
TCTCAACTTGCACCCCGAATTCCACAGGTTTCCTCCCACAGGCTACTTCAAGCAAGAAAA
CACCGAAAGCATATACATCGGTTATAGTGGAAGCTCCCATTGTTATTAGCTCAGGTGCCA
TGTACCCTACAGTGCCAACCGCTGCTGTTGTGATATATTCAATTGTAAATGGCTTCATGT
CCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAG
TAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGA
AAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAA
ACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTACATTCA

Last Updated on 06.12.2018 16:11