Sequences from confirmation amplicon


Confirmation sequences for line 779B03 and hit At4g31940 are shown.
Line ID 779B03
Locus At4g31940 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:15453387
Primers used for PCR 19kw - 8474, show primer details
   
Sequence Name 45-K113872-0022-779-B03C-19kw
GenBank Accession LN505603 [GenBank]
Sequencing primer gene specific primer, 19kw: 5'-AAACTCTCGCATCTCCAGTATGAT-3'
Sequence (A. th genome BLAST matches underlined) 
>45-K113872-0022-779-B03C-19kw
CTTTCTATCCAAATCTTGATTTTAAGAAAAAAATATGAGATAACTAAAATACTTACCAAA
TTGGGATTTAACCCAAAATATAATGTTTTATAATATATAAGTTTAACTAGAAATGTTAGG
TGGAGAGACGCACTTGGGTCATATTTAAAAGAAATCAATTACAACCTAAAATGTGATGTG
GAGATTAATTAGATGATATTGACTCTTTTAATGTGTGTGATATAAATATACTATGAATAA
TCAGGCACTGATTCTTGGAGGAAGTGACACTTCAGCATCAACCCTTACATGGAGGATATA
TTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGG
TCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATG
TCCGCACGGTTATT
   
Sequence Name 19-K113871-0022-779-B03C-8409
GenBank Accession LN494844 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>19-K113871-0022-779-B03C-8409
TTACATTGAATAATCCTCCATGTAAGGGTTGATGCTGAAGTGTCACTTCCTCCAAGAATC
AGTGCCTGATTATTCATAGTATATTTATATCACACACATTAAAAGAGTCAATATCATCTA
ATTAATCTCCACATCACATTTTAGGTTGTAATTGATTTCTTTTAAATATGACCCAAGTGC
GTCTCTCCACCTAACATTTCTAGTTAAACTTATATATTATAAAACATTATATTTTGGGTT
AAATCCCAATTTGGTAAGTATTTTAGTTATCTCATATTTTTTTCTTAAAAATCAAGATTT
GTGTATAGAAAGGTATACCAGGCAGGTAGATTTGATGCTAGTATTTGCATCATACTGGAG
ATGGGAGAGTT

Last Updated on 06.12.2018 16:11