Sequences from confirmation amplicon


Confirmation sequences for line 779B11 and hit At1g33360 are shown.
Line ID 779B11
Locus At1g33360 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:12092311
Primers used for PCR 39ci - 8474, show primer details
   
Sequence Name 28-K107934-0022-779-B11C-39ci
GenBank Accession LN498877 [GenBank]
Sequencing primer gene specific primer, 39ci: 5'-CTTCACTTTCTCCGTTTTTATCGT-3'
Sequence (A. th genome BLAST matches underlined) 
>28-K107934-0022-779-B11C-39ci
GATGGCTTTTTAGGTTTCCCCACTCGATCTAATCTAACCTAACGCTCCTTCATTAACTTT
CTTCAGACAGATTACAATAACGTGTAATATACAAGACACAGAAACATCATTGATTCCGAT
CTCAAGTTTTGTCTTTTTGATTTCATATACACAGATACATAGATAACAAACACACATCTT
TGATCAAATGTCTGGACTTTGGAGATTGAGGAATCTAAAGTCTCTAGCTTTACATGCTCG
TTCGATTTCACCTGTTTCCAATCTTTATTCTCTGGAATTAGGATCATGCCCTCGTCGTCG
TATTCAAGAACGATTCAAATCGGAACAAGGCGGCGGAGGAGGAGGAGGAGATGATTTTCC
GGTTCCGGTGACTCGTCGGAAGTTAAGATCTTCAAATGGCTTCATGTCCGGGAAATCTAC
ATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTT
TTTTTCAATTCAAAAATGTAGATGTCCGCACGNTTATTA
   
Sequence Name 12-K107933-0022-779-B11C-8409
GenBank Accession LN491610 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>12-K107933-0022-779-B11C-8409
GCCTTTGAGATCTTAACTTCCGACGAGTCACCGGAACCGGAAAATCATCTCCTCCTCCTC
CTCCGCCGCCTTGTTCCGATTTGAATCGTTCTTGAATACGACGACGAGGGCATGATCCTA
ATTCCAGAGAATAAAGATTGGAAACAGGTGAAATCGAACGAGCATGTAAAGCTAGAGACT
TTAGATTCCTCAATCTCCAAAGTCCAGACATTTGATCAAAGATGTGTGTTTGTTATCTAT
GTATCTGTGTATATGAAATCAAAAAGACAAAACTTGAGATCGGAATCAATGATGTTTCTG
TGTCTTGTATATTACACGTTATTGTAATCTGTCTGAAGAAAGTTAATGAAGGAGCGTTAG
GTTAGATTAGATCGAGTGGGGAAACCTTAAAAAGCCATCAATTTGGAGAAGAAGAAGAGA
AACGATAAAAACGGAGAAAGTGAAG

Last Updated on 06.12.2018 16:11