Sequences from confirmation amplicon


Confirmation sequences for line 779D05 and hit At5g55920 are shown.
Line ID 779D05
Locus At5g55920 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:22645951
Primers used for PCR 30ew - 8474, show primer details
   
Sequence Name 57-K110987-0022-779-D05C-30ew
GenBank Accession LN509451 [GenBank]
Sequencing primer gene specific primer, 30ew: 5'-CGTCGTGAATAAACAAAAACAAGA-3'
Sequence (A. th genome BLAST matches underlined) 
>57-K110987-0022-779-D05C-30ew
AGATGATAACGTTTTCGCAAATGTTTTTTCTTCTCTTTGATGAAAAAACACTGAATCATC
ATTACTTCTCCTCCTTCGCCTTCTCCCGCATGGCTATTCTTTTCTCTTCCCTGCAAACGT
GATTATACAAATCATTGTCAAGCAACTATATATAAAAGACAAGTCTAAAGCATTTTGTTC
TCTCAGTTCATGGATGGGGATAGATATTTATATACCTAGCTTGAGCTATTTCGTTCTTCC
ATTCCCGTCTCTTCTTCTTTTGCTTCCTTGGCTTCATGTAAATGGCTTCATGTCCGGGAA
ATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTAC
CAATTTTTTTTCAATTCAAAAATGTAGATGTCAGCAACGTTATT
   
Sequence Name 41-K110986-0022-779-D05C-8409
GenBank Accession LN504116 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>41-K110986-0022-779-D05C-8409
ATGAAGCCAAGGAAGCAAAAGAAGAAGAGACGGGAATGGAAGAACGAAATAGCTCAAGCT
AGGTATATAAATATCTATCCCCATCCATGAACTGAGAGAACAAAATGCTTTAGACTTGTC
TTTTATATATAGTTGCTTGACAATGATTTGTATAATCACGTTTGCAGGGAAGAGAAAAGA
ATAGCCATGCGGGAGAAGGCGAAGGAGGAGAAGTAATGATGATTCAGTGTTTTTTCATCA
AAGAGAAGAAAAAACATTTGCGAAAACGTTATCATCTATGGTTATAGTAGGCTTGTGCCA
TATATTGGCTTTCTTGTTTTTGTTTATTCACGACG

Last Updated on 06.12.2018 16:11