Sequences from confirmation amplicon


Confirmation sequences for line 779E04 and hit At3g17940 are shown.
Line ID 779E04
Locus At3g17940 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:6143749
Primers used for PCR 62sm - 8474, show primer details
   
Sequence Name 47-K116491-0022-779-E04C-62sm
GenBank Accession LN506309 [GenBank]
Sequencing primer gene specific primer, 62sm: 5'-TTAAGGGAGGACGAAGAGATTACA-3'
Sequence (A. th genome BLAST matches underlined) 
>47-K116491-0022-779-E04C-62sm
CTTTTGCTTCTTAGTGGCGCGTGTTTCTCACCGCCCAAAAGACATGCGTGTAGACATAAT
TGCCATCAGAATTACTCAAATACACTAATTGCTCTTTTTTTCCAAGTTCATGTGAACAAT
GGGAGATTCATTCGCTGCACAATTCACAATTTCACAAATGGGCCAAATGTTTAGAAGATA
TGAAAAAATAAAGTAACTTTAATATAATGTAAACAGTTTGGTTTTATTATTCTCCCCCAA
AGAGATAAAATTAAAATCACTCTTTTTCCAGCCCAGTGAATCAAATGAACCGGCCCTTTA
TTCCCATCATCTAACACCTAAAACGTCCATCTATAAACTCAAGCTGAAAACTCAAACAGC
ATCGTGTGATTGTACTTCTCATCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGA
AATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTA
CCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCA
   
Sequence Name 15-K116490-0022-779-E04C-8409
GenBank Accession LN493138 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>15-K116490-0022-779-E04C-8409
ATTGAATATATCCTGATGAGAAGTACAATCACACGATGCTGTTTGAGTTTTCAGCTTGAG
TTTATAGATGGACGTTTTAGGTGTTAGATGATGGGAATAAAGGGCCGGTTCATTTGATTC
ACTGGGCTGGAAAAAGAGTGATTTTAATTTTATCTCTTTGGGGGAGAATAATAAAACCAA
ACTGTTTACATTATATTAAAGTTACTTTATTTTTTCATATCTTCTAAACATTTGGCCCAT
TTGTGAAATTGTGAATTGTGCAGCGAATGAATCTCCCATTGTTCACATGAACTTGGAAAA
AAAGAGCAATTAGTGTATTTGAGTAATTCTGATGGCAATTATGTCTACACGCATGTCTTT
TGGGCGGTGAGAAACACGCGCCACTAAAGAAGCAAAAGCCTATGGGCCCCAAAAAATATC
AACCATCCATCTCTGTAATCTCTTCGCCTTCCCTTAAA

Last Updated on 06.12.2018 16:11