Sequences from confirmation amplicon


Confirmation sequences for line 780G09 and hit At4g34590 are shown.
Line ID 780G09
Locus At4g34590 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:16522860
Primers used for PCR MR84 - 8474, show primer details
   
Sequence Name 45-K027791-022-780-G09C-8409
GenBank Accession LN505407 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>45-K027791-022-780-G09C-8409
AGCCATTTACATCACGAAGAAATCATCACACTCCAAACCAACCAGAGGGTTCGAACACAT
GCCCATGTTGTTGTTGTTGTTGTTGTTGCTACTGTCGAGGAACTCGATGATGTCGTTGAG
AGATTGGAGCCTGTGGTTAAGTTCATCAAGCTGAGCTCTGAGAACAGAGTTCTCTGCTTC
AACGGTTAGGTAGTGTTGCGTTGTGATGCTGACACTTGTCACGATCTCCGTGTTCTCTTT
TTTCAGATGATTAACCTGAGCCGTTAGATCGTCTAGGAGCTTTTGTTTCTTCATTCTTGA
TCTCCTTGCAGATTCACGGTTTGAGAGCATCCGTTTACGTTTCCTCTGCTCCATGAGACT
CTCCTCCGATCCTGACGACGTTTGAATCGTCGACGAAGTTGTTCCCGACGACGACGATTC
CATTTAGTAACACACAAACAAAAACAGGGAAGAGTTTTTTTTAAGAGAAGAGAGATAAAA
TATTTTAAAATGGGTTTTTAGGGGAATTAAAAACAGAACAGAACAAGACACAACAGAACA
GGACAGGACCCAGATGACG
   
Sequence Name 46-K027791-022-780-G09C-MR84
GenBank Accession LN505749 [GenBank]
Sequencing primer gene specific primer, MR84: 5'-CAAATTTCTCTTCTTCGTTTTTCG-3'
Sequence (A. th genome BLAST matches underlined) 
>46-K027791-022-780-G09C-mr84
GTCTTGTTCTGTTCTGTTTTTAATTCCCCTAAAAACCCATTTTAAAATATTTTATCTCTC
TTCTCTTAAAAAAAACTCTTCCCTGTTTTTGTTTGTGTGTTACTAAATGGAATCGTCGTC
GTCGGGAACAACTTCGTCGACGATTCAAACGTCGTCAGGATCGGAGGAGAGTCTCATGGA
GCAGAGGAAACGTAAACGGATGCTCTCAAACCGTGAATCTGCAAGGAGATCAAGAATGAA
GAAACAAAAGCTCCTAGACGATCTAACGGCTCAGGTTAATCATCTGAAAAAAGAGAACAC
GGAGATCGTGACAAGTGTCAGCATCACAACGCAACACTACCTAACCGTTGAAGCAGAGAA
CTCTGTTCTCAGAGCTCAGCTTGATGAACTTAACCACAGGCTCCAATCTCTCAACGACAT
CATCGAGTTCCTCGACAGTAGCAACAACAACAACAACAACAACATGGGCATGTGTTCGAA
CCCTCTGGTTGGTTTGGAGTGTGATGATTTCTTCGTGATTGTAAATGGCTTCATGTCCGG
GAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAAT
TACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGT
ACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAA
ATTATTCTAATTCGGAAATCTTATTTCGACGTGTCTAC

Last Updated on 06.12.2018 16:11