Sequences from confirmation amplicon


Confirmation sequences for line 781D02 and hit At2g15000 are shown.
Line ID 781D02
Locus At2g15000 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:6482367
Primers used for PCR NF25 - 8474, show primer details
   
Sequence Name 09-K102429w-0022-781-D02C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>09-K102429w-0022-781-D02C-8409
ATTTACATGGATCAGAGGTTTAGGGAAACCCTAGCAAAAACCTCTCTCTCTCTCTCAATT
CACTTCTCCTCCTTCAATGGCTTGGCGCAACGCAGGATCTGCCGCTCGTTCTTTCGTCTC
CGCCACCGCAAGATCACCATCTCTCCGTTCTCCCACCACGGCGCTTCCTCGCCTCCGTCC
TCCTCAATCCTCCTTACCTAGCCGTCGCTTCACCTTCTCATCACCTTCCAGGTTCGTGAA
CCTAACTCCTTCACAGTCTCTTTATCAAATTTCAACAAAATCCAATCAATTCAGTGAGAA
TTAAAAATCTTCGTATCTCGATTTTGAAAATTTTGAGATTCGTAGCTTCCTTAGATCTCT
AATTGGAAAGAATCTGATGTTGATTTGTGAATATCTTCTTCAGGAATCTAGGAGCA
   
Sequence Name 25-K102430-0022-781-D02C-NF25
GenBank Accession LN497384 [GenBank]
Sequencing primer gene specific primer, NF25: 5'-GTGCTCCTAGATTCCTGAAGAAGA-3'
Sequence (A. th genome BLAST matches underlined) 
>25-K102430-0022-781-D02C-NF25
TTTCCATAGAGATCTAGGAAGCTACGAATCTCAAAATTTTCAAAATCGAGATACGAAGAT
TTTTAATTCTCACTGAATTGATTGGATTTTGTTGAAATTTGATAAAGAGACTGTGAAGGA
GTTAGGTTCACGAACCTGGAAGGTGATGAGAAGGTGAAGCGACGGCTAGGTAAGGAGGAT
TGAGGAGGACGGAGGCGAGGAAGCGCCGTGGTGGGAGAACGGAGAGATGGTGATCTTGCG
GTGGCGGAGACGAAAGAACGAGCGGCAGATCCTGCGTTGCGCCAAGCCATTGAAGGAGGA
GAAGTGAATTGAGAGAGAGAGAGAGGTTTTTGCTAGGGTTTCCCTAAACCTCTGATCCAT
GTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATAT
GGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAG
CGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTA
TAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTACA
TTCACTCCCAAATGG

Last Updated on 06.12.2018 16:11