Sequences from confirmation amplicon


Confirmation sequences for line 781D10 and hit At5g09420 are shown.
Line ID 781D10
Locus At5g09420 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:2929783
Primers used for PCR 25an - 8474, show primer details
   
Sequence Name 06-K106755-0022-781-D10C-8409
GenBank Accession LN488830 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>06-K106755-0022-781-D10C-8409
GCCTTTACATTGAATATACATGGATTTTCCGCAAGACTTGGTACATTTGAAGCGACATAT
TGGCCAACGTTAACATGTTTTGGAGTCTTGTCTGCATGAGCCAGTGGTCAAGAGACATAA
GATATGAATCTGGAGAAGGGCAAAGAGAAGGAAACAATACAGCTGCAAACTTACAGCCAG
ATAGATTCTCGATTGCCTTTCTGACAACTTGCACCGACTTTTGCTTAGGAATGTCAGAGA
GCTCGAATAAATCATCAGCAAAGATTAAAGATCTTTGCCTTCTATGTGTTACTGCACTCA
GATTTAGTAAGGCATGTCCAACTTGACACAAAACGGATGGATCAGAGGCAAACCATCCTA
CAGAACGAATCAATGAGTTCAATAATCAGGAAGGCAAACACCTAGAGTAGCATGTACCAT
TGAAACTACACTAAAAACACTAAAAGGGAGGGGAATGTAACGTACCAACAGTCTCTAGGC
TCTGAGAATTGGGCAAAACTCCAACTGAAGAAACAGTCCCCTGAGATGGTCGAAAACCAA
GAATACCACAAAAGGCTGCTGGGACTCTAACACCACCAGTGGTATCAATCCCTGCAAATC
AAGCATCTTTTGATTAGCATAAAGAACCAAACGTAT
   
Sequence Name 22-K106756-0022-781-D10C-25an
GenBank Accession LN496132 [GenBank]
Sequencing primer gene specific primer, 25an: 5'-GTGTTTCTTGGATTACGTTTGGTT-3'
Sequence (A. th genome BLAST matches underlined) 
>22-K106756-0022-781-D10C-25an
TGATACCACTGGTGGTGTTAGAGTCCCAGCAGCCTTTTGTGGTATTCTTGGTTTTCGACC
ATCTCAGGGGACTGTTTCTTCAGTTGGAGTTTTGCCCAATTCTCAGAGCCTAGAGACTGT
TGGTACGTTACATTCCCCTCCCTTTTAGTGTTTTTAGTGTAGTTTCAATGGTACATGCTA
CTCTAGGTGTTTGCCTTCCTGATTATTGAACTCATTGATTCGTTCTGTAGGATGGTTTGC
CTCTGATCCATCCGTTTTGTGTCAAGTTGGACATGCCTTACTAAATCTGAGTGCAGTAAC
ACATAGAAGGCAAAGATCTTTAATCTTTGCTGATGATTTATTCGAGCTCTCTGACATTCC
TAAGCAAAAGTCGGTGCAAGTTGTCAGAAAGGCAATCGAGAATCTATCTGGCTGTAAGTT
TGCAGCTGTATTGTTTCCTTCTCTTTGCCCTTCTCCAGATTCATATCTTATGTCTCTTGA
CCACTGGCTCATGCAGACAAGACTCCAAAACATGTTAACGTTGGCCAATATGTCGCTTCA
AATGTACCAAGTCTTGCGGAAAATCCATGTATATTCAATTGTAAATGGCTTCATGTCCGG
GAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAAT
TACCAATTTTTTTTCAATTCAAAAATGAAGATGTCCC
Primers used for PCR 27au - 8474, show primer details
   
Sequence Name 04-K106867-0022-781-D10C-8409
GenBank Accession LN487939 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>04-K106867-0022-781-D10C-8409
ATTGCTGATTATTTTTGAAATATCCATGTATATTCTGTGAATATATCCCAAAATTCAGCA
TCAACTTTAAGGGCTCTTTCATCTGTGATGTTGGCTATACAAAGGTATTTAAGAAGCTCA
CACTTCTTGAAATTTTCACTGCATCAATACTTTTTTCTCTCTCTCGTTCCTTTTAAACGG
GTGCAATAAAAGATTGTTGTTATTCCCTCTTTGCTGGAGGTTACTATTTTCTTCTTAAGT
TGTATTATATGCAAATATCGAAGAAGAAAGGATTTTCTTTTTACCAGGCATGAGTTCAAA
ACAAATCATGAAGAATGGTGGCAAACATGCAAATCGTTTCTAGGGCCAAGATTTTCCAAT
GATGTTGTTACAGCTCTGAAGAGC
   
Sequence Name 14-K106868-0022-781-D10C-27au
GenBank Accession LN492527 [GenBank]
Sequencing primer gene specific primer, 27au: 5'-TGCTCTTCAGAGCTGTAACAACAT-3'
Sequence (A. th genome BLAST matches underlined) 
>14-K106868-0022-781-D10C-27au
GATTTGCATGTTTGCCACCATTCTTCATGATTTGTTTTGAACTCATGCCTGGTAAAAAGA
AAATCCTTTCTTCTTCGATATTTGCATATAATACAACTTAAGAAGAAAATAGTAACCTCC
AGCAAAGAGGGAATAACAACAATCTTTTATTGCACCCGTTTAAAAGGAACGAGAGAGAGA
AAAAAGTATTGATGCAGTGAAAATTTCAAGAAGTGTGAGCTTCTTAAATACCTTTGTATA
GCCAACATCACAGATGAAAGAGCCCTTAAAGTTGATGCTGAATTTTGGGATATATTCACA
GAATATACATGGATATATTCAAAAATGAATCAGCAATTCAATTGTAAATGGCTTCATGTC
CGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGT
AATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGC
Primers used for PCR 09mg - 8474, show primer details
   
Sequence Name 82-K118414-0022-781-D10C-09mg
GenBank Accession LN514239 [GenBank]
Sequencing primer gene specific primer, 09mg: 5'-TTGATACCACTGGTGGTGTTAGAG-3'
Sequence (A. th genome BLAST matches underlined) 
>82-K118414-0022-781-D10C-09mg
TCTCAGGGGACTGTTTCTTCAGTTGGAGTTTTGCCCAATTCTCAGAGCCTAGAGACTGTT
GGTACGTTACATTCCCCTCCCTTTTAGTGTTTTTAGTGTAGTTTCAATGGTACATGCTAC
TCTAGGTGTTTGCCTTCCTGATTATTGAACTCATTGATTCGTTCTGTAGGATGGTTTGCC
TCTGATCCATCCGTTTTGTGTCAAGTTGGACATGCCTTACTAAATCTGAGTGCAGTAACA
CATAGAAGGCAAAGATCTTTAATCTTTGCTGATGATTTATTCGAGCTCTCTGACATTCCT
AAGCAAAAGTCGGTGCAAGTTGTCAGAAAGGCAATCGAGAATCTATCTGGCTGTAAGTTT
GCAGCTGTATTGTTTCCTTCTCTTTGCCCTTCTCCAGATTCATATCTTATGTCTCTTGAC
CACTGGCTCATGCAGACAAGACTCCAAAACATGTTAACGTTGGCCAATATGTCGCTTCAA
ATGTACCAAGTCTTGCGGAAAATCCATGTATATTCAATTGTAAATGGCTTCATGTCCGGG
AAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATT
ACCAATTTTTTTTCAATTCAAAAATGTAGATATCCGCA
   
Sequence Name 34-K118413-0022-781-D10C-8409
GenBank Accession LN501597 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>34-K118413-0022-781-D10C-8409
TTGAATATACATGGATTTTCCGCAAGACTTGGTACATTTGAAGCGACATATTGGCCAACG
TTAACATGTTTTGGAGTCTTGTCTGCATGAGCCAGTGGTCAAGAGACATAAGATATGAAT
CTGGAGAAGGGCAAAGAGAAGGAAACAATACAGCTGCAAACTTACAGCCAGATAGATTCT
CGATTGCCTTTCTGACAACTTGCACCGACTTTTGCTTAGGAATGTCAGAGAGCTCGAATA
AATCATCAGCAAAGATTAAAGATCTTTGCCTTCTATGTGTTACTGCACTCAGATTTAGTA
AGGCATGTCCAACTTGACACAAAACGGATGGATCAGAGGCAAACCATCCTACAGAACGAA
TCAATGAGTTCAATAATCAGGAAGGCAAACACCTAGAGTAGCATGTACCATTGAAACTAC
ACTAAAAACACTAAAAGGGAGGGGAATGTAACGTACCAACAGTCTCTAGGCTCTGAGAAT
TGGGCAAAACTCCAACTGAAGAAACAGTCCCCTGAGATGGTCGAAAACCAAGAATACCAC
AAAAGGCTGCTGGGACTCTAACACCACCAGGGGGTATC

Last Updated on 06.12.2018 16:11