Sequences from confirmation amplicon


Confirmation sequences for line 782D07 and hit At1g53880 are shown.
Line ID 782D07
Locus At1g53880 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:20116356
Primers used for PCR 22zg - 8474, show primer details
   
Sequence Name 23-K125692-0022-782-D07C-22zg
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, 22zg: 5'-TAAGATTGTAAAACGCAAATCGGT-3'
Sequence (A. th genome BLAST matches underlined) 
>23-K125692-0022-782-D07C-22zg
TGGCAGAAGTTAAAAGAAACATCAAATATCAAAAGTTTAGATCTTGGGAAAGATGTATTA
ACAATGATGGTGGAGCCACAAGTAGATCACTCTTTAATAATGGGTATTGTTATAGCATAT
AGTCTTCTCAAATGTAAATTGTGATAATTAGTAGGGGTGAGATTGAACTATTGTGTAAAC
TTTAGGGTCGTAAATGTAAATATACAATTTTGGAGAGCCAAATGATAATATTATTGTTAA
TGAAGTTCTTAAAGCTAAATTAAATGTTATTACAGGCATGAGGCATAAACCCCAAAGTCT
CTAGCTTGAAACTGAGATTTTCGAAGAATTCATCAAAATGTGGAGAGAATCGGCTTCGTT
TATCCTCGATAAGCATCAGAACAACAAACCCATTTCTCTAACTCATTCAATTGATTCTCC
TCCACCACCATCTGCTTCAATGGCGGATGAAAACCCTAACCCTAATCCTATTTCGGCTTA
TTACCAAACCAGAGCGGCGCATCATGGAATTGTGACTAGCGAGTGGTTGGAGCAGGCTCA
AGCCGCGGTTCGACGTTACCCTGATCGTGATTCCTTAGTTTCGGGAAGACCCTTTAGCGT
GATTGAGGACTTTAATAGTTGGAGACAGCAACCTGATTTGGCGGAGGCTGTTGCGGCGAT
TCGTGCTCTTGCGGCTGTTATTAGGGCTAGTGAAGCTACTACTATGATGGAACTTGAAAT
TGAACTCAAGAAAAAGTATGATGGCTATATTCAATTGTAAATGGCTTCATGTCCGGGAAA
TCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACC
AATTTTTTTTCAAT
   
Sequence Name 07-K125691-0022-782-D07C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>07-K125691-0022-782-D07C-8409
CATTTCATTGAAATAGCCATCATACTTTTTCTTGAGTTCAATTTCAAGTTCCATCATAGT
AGTAGCTTCACTAGCCCTAATAACAGCCGCAAGAGCACGAATCGCCGCAACAGCCTCCGC
CAAATCAGGTTGCTGTCTCCAACTATTAAAGTCCTCAATCACGCTAAAGGGTCTTCCCGA
AACTAAGGAATCACGATCAGGGTAACGTCGAACCGCGGCTTGAGCCTGCTCCAACCACTC
GCTAGTCACAATTCCATGATGCGCCGCTCTGGTTTGGTAATAAGCCGAAATAGGATTAGG
GTTAGGGTTTTCATCCGCCATTGAAGCAGATGGTGGTGGAGGAGAATCAATTGAATGAGT
TAGAGAAATGGGTTTGTTGTTCTGATGCTTATCGAGGATAAACGAAGCCGATTCTCTCCA
CATTTTGATGAATTCTTCGAAAATCTCAGTTTCAAGCTAGAGACTTTGGGGTTTATGCCT
CATGCCTGTAATAACATTTAATTTAGCTTTAAGAACTTCATTAACAATAATATTATCATT
TGGCTCTCCAAAATTGTATATTTACATTTACGACCCTAAAGTTTACACAATAGTTCAATC
TCACCCCTACTAATTATCACAATTTACATTTGAGAAGACTATATGCTATAACAATACCCA
TTATTAAAGAGTGATCTACTTGTGGCTCCACCATCATTGTTAATACATCTTTCCCAAGAT
CTAAACTTTTGATATTTGATTGTTTCTTTTTAACTTCTGCCAATAATCTTCCTGATCCAT
CCTCGATTGTGAAAACCGATNTGCGTT

Last Updated on 06.12.2018 16:11