Sequences from confirmation amplicon


Confirmation sequences for line 782D10 and hit At3g13445 are shown.
Line ID 782D10
Locus At3g13445 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:4381727
Primers used for PCR 53fr - 8474, show primer details
   
Sequence Name 29-K111563-0022-782-D10C-53fr
GenBank Accession LN499348 [GenBank]
Sequencing primer gene specific primer, 53fr: 5'-GGGCTACACACTGAGTTAAGGTTC-3'
Sequence (A. th genome BLAST matches underlined) 
>29-K111563-0022-782-D10C-53fr
AGCAACATACATGTAAGACATATCTTGGAAAGAAAAAGAAAAAAAATAGAACCGAAGAAT
TGTCTTTAGAATCTCCATTGTGTATATACAATGTTATATTATGAACCAAAACACAAGGTG
AGAGTCTAGCTACTGTGAAAGCCTTTGTTCAAGGGCAAAATTCTCAGCAAGCTTTAGCAA
CTCCAAGGGGCTAAGACCGCACAACTAGTTTTGCAGGGAACAAATCCGTGACCCTATAAA
AGATAAACACAACAATTAGGTTCATGCACGAATAAATCGAGAAATCAAACATTACTAAGA
AAGATGAGGAAAGTTGACGGGTGTGTTACATACTATTGCTGTATCTTTCTGAATTCCGAG
AGCACGGGGTATATTACTACAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAAT
CTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCA
ATTTTTTTTCAATTCAAAAATGTAGATGTCCG
   
Sequence Name 13-K111556w-0022-782-D10C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>13-K111556w-0022-782-D10C-8409
CATTTCATTGAATTATCCTGTAGTATATACCCCGTGCTCTCGGAATTCAGAAAGATACAG
CAATAGTATGTAACACACCCGTCAACTTTCCTCATCTTTCTTAGTAATGTTTGATTTCTC
GATTTATTCGTGCATGAACCTAATTGTTGTGTTTATCTTTTATAGGGTCACGGATTTGTT
CCCTGCAAAACTAGTTGTGCGGTCTTAGCCCCTTGGAGTTGCTAAAGCTTGCTGAGAATT
TTGCCCTTGAACAAAGGCTTTCACAGTAGCTAGACTCTCACCTTGTGTTTTGGTTCATAA
TATAACATTGTATATACACAATGGAGATTCTAAAGACAATTCTTCGGTTCTATTTTTTTT
CTTTTTCTTTCCAAGATATGTCTTACATGTATGTTGCTGTAGTGTCCTCACATTTCCACT
TATGTTACAAGTAGAACCTTAACTCATGATGTAGCC

Last Updated on 06.12.2018 16:11