Sequences from confirmation amplicon


Confirmation sequences for line 783A03 and hit At5g08340 are shown.
Line ID 783A03
Locus At5g08340 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:2684823
Primers used for PCR 18xb - 8474, show primer details
   
Sequence Name 34-K119373-0022-783-A03C-18xb
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, 18xb: 5'-ACCTTTCTACTCTTTGATGGGGAT-3'
Sequence (A. th genome BLAST matches underlined) 
>34-K119373-0022-783-A03C-18xb
TATACATTTGGTAGAAGAATAGATGTTAGCTCATGATCATCTGATGCATAATATGTTGCA
GGAGGCATTGTTGCTCTAGGGAAGTTTGATGCCTTACATATCGGCCATCGAGAGCTTACA
ATTCAAGCTTCAAGAATTGGAGCTCCATATCTATTATCTTTTGTTGGAATGGCCGAGGTA
CTTGGTTGGGAGCCCAGGTAATGTCCCCACCTGTAGTATTCATGTCTTCCCTTTCTCTTT
TCCAACCTAAGATTCTTGTTCGTTTGAGATGGTTAAACATTATATATGATCCTGCTGATG
AAGTTCGTTTTTTTTAATCAATATGTGCTCTCTTCCATTGTGACTACTGACAATCAGGAT
ATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGA
TGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAG
ATATCCTAAGGTTTTTTA
   
Sequence Name 02-K119372-0022-783-A03C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>02-K119372-0022-783-A03C-8409
ATTGAATATATCCTGATTGTCAGTAGTCACAATGGAAGAGAGCACATATTGATTAAAAAA
AACGAACTTCATCAGCAGGATCATATATAATGTTTAACCATCTCAAACGAACAAGAATCT
TAGGTTGGAAAAGAGAAAGGGAAGACATGAATACTACAGGTGGGGACATTACCTGGGCTC
CCAACCAAGTACCTCGGCCATTCCAACAAAAGATAATAGATATGGAGCTCCAATTCTTGA
AGCTTGAATTGTAAGCTCTCGATGGCCGATATGTAAGGCATCAAACTTCCCTAGAGCAAC
AATGCCTCCTGCAACATATTATGCATCAGATGATCATGAGCTAACATCTATTCTTCTACC
AAATGTATACTAAAGCTCTTTCCAAAAGGAAAGGAACCGCACAGTAACCACAAAATCCCC
ATCAAAAGAAAGAAAGG

Last Updated on 06.12.2018 16:11