Sequences from confirmation amplicon


Confirmation sequences for line 783C02 and hit At3g49810 are shown.
Line ID 783C02
Locus At3g49810 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:18475082
Primers used for PCR HM14 - 8474, show primer details
   
Sequence Name 03-K029007-022-783-C02C-8409
GenBank Accession LN487352 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>03-K029007-022-783-C02C-8409
GACCAACCTCAACAGCAAGTGGTGAAACATTTCTTCTGGAGCTAATTTGCAGAACAGACC
AAAGAATCGACAGCGCGTAGTTAGTGCAATTCTCAGACACCCTCATCAGCACCCTAACTG
TATAAGGTATTGTGTTTGCAGAGTCTTTGACAGCAACTCTTCCTTCCACGTCTGTACACA
AAGCATCAAGAACAAACAGAGCTAACTCCAAACATTCCGGGTCTAAAGACGGTAAGATAT
CAACTAATTGAGGCACTGCTCCAATGCTAACCATCAAGCTTCGAACTTGTTTATGAACCG
AAATCGGTTTAAGCAACCGAAGTGCAGGAGATACTCCATTTCTATGTCTCTTATCCTTAA
CCAATCTCATTANCNCCAACA
   
Sequence Name 04-K029007-022-783-C02C-HM14
GenBank Accession LN487787 [GenBank]
Sequencing primer gene specific primer, HM14: 5'-CTTGTTGGGTTAATGAGATTGGTT-3'
Sequence (A. th genome BLAST matches underlined) 
>04-K029007-022-783-C02C-HM14
CTTACCGTCTTTAGACCCGGAAACGTTTGGAGTTAGCTCTGTTTCGTTCTCGATGCTTTG
TGTACAGACGTGGAAGGAAGAGTTGCTGTCAAAGACTCTGCAAACACAATACCTTATACA
GTTAGGGTGCTGATGAGGGTGTCTGAGAATTGCACTAACTACGCGCTGTCGATTCTTTGG
TCTGTCTGCAAATTAGCTCCAGAAGAATGTTCACCACTTGCTGTTGAGGTTGGTCTTGCT
GCAAAGCTGTTACTCGTGATCCGGGAAATATGTTACTATGGATCAGCAATGAGTATGATG
GTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGAT
GTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGT
CGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACG
TGTCTACAT

Last Updated on 06.12.2018 16:11