Sequences from confirmation amplicon


Confirmation sequences for line 784G09 and hit At2g30590 are shown.
Line ID 784G09
Locus At2g30590 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:13033631
Primers used for PCR IS73 - 8474, show primer details
   
Sequence Name 27-K026485-022-784-G09C-8409
GenBank Accession LN498227 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>27-K026485-022-784-G09C-8409
GATTTCCCGGACATGAAGCCATTTACAATTGCTGACCCTCTTTTTAGCTCTACAAGCATC
CAAGGAACCCCTCTTACTTTTCCCTCTTCTTCATTCCCTCTCTCTCTATATCTCCCAATT
CCTTCTCTTTTTTTAACCTTGATCTTCTTCTTATAAGAGACTCAGAGCTGTTCTTCAAGA
TGGGATTGTGTAAGCTTGAAACTTGACTATCTGGGTCTCTCCCATTTTCTGTTCTCTTTT
GTATTCTGAGTGAAAGTTTTGTTTTCTGGGGGAAAAAATTAGGGTTCTTAGATGGAGGAG
ATAGAAGGAACAAACAGAGCAGCTGTTGAGAGTTGTCATAGAGTTCTTAATCTTTTACAT
AGATCACAGCAACAAGATCATGTTGGTTTTGAAAAGAATTTAGTATCTGAAACTAGAGAA
GCTGTGATTAGGTTCAAGAGAGTTGGGAGTTTGTTAAGCAGTAGTGTTGGTCATGCTAGG
TTTAGAAGAGCTAAGAAACTTCAGAGTCATGTCTCTCAAAGTCTCTTACTTGATCCATGT
CAACAAAGGACAACAGAAGTTCCATCATCATCTTCTCAGAAAACACCGGTACTCCGGTCT
GGTTTCCAGGGAATTGAGCTTGAGACAACCTTCAGA
   
Sequence Name 28-K026485-022-784-G09C-IS73
GenBank Accession LN498694 [GenBank]
Sequencing primer gene specific primer, IS73: 5'-CCCTAAAGTGAGTGAATCTGAAGG-3'
Sequence (A. th genome BLAST matches underlined) 
>28-K026485-022-784-G09C-IS73
GCTCAATTCCTGGAAACCAGACCGGAGTACCGGTGTTTTCTGAGAAGATGATGATGGAAC
TTCTGTTGTCCTTTGTTGACATGGATCAAGTAAGAGACTTTGAGAGACATGACTCTGAAG
TTTCTTAGCTCTTCTAAACCTAGCATGACCAACACTACTGCTTAACAAACTCCCAACTCT
CTTGAACCTAATCACAGCTTCTCTAGTTTCAGATACTAAATTCTTTTCAAAACCAACATG
ATCTTGTTGCTGTGATCTATGTAAAAGATTAAGAACTCTATGACAACTCTCAACAGCTGC
TCTGTTTGTTCCTTCTATCTCCTCCATCTAAGAACCCTAATTTTTTCCCCCAGAAAACAA
AACTTTCACTCAGAATACAAAAGAGAACAGAAAATGGGAGAGACCCAGATAGTCAAGTTT
CAAGCTTACACAATCCCATCTTGAAGAACAGCTCTGAGTCTCTTATAAGAAGAAGATCAA
GGTTAAAAAAAGAGAAGGAATTGGGAGATATAGAGAGAGAGGGAATGAAGAAGAGGGAAA
AGTAAGAGGGGTTCCTTGGATGCTTGTAGAGCTAAAAAGAGGGTCAGGCAATTGTAAATG
GCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAA
AAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATT
ATAAAATGAAAGT

Last Updated on 06.12.2018 16:11