Sequences from confirmation amplicon


Confirmation sequences for line 784H03 and hit At1g01420 are shown.
Line ID 784H03
Locus At1g01420 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:155551
Primers used for PCR AB24 - 8474, show primer details
   
Sequence Name 15-K103785-0022-784-H03C-AB24
GenBank Accession LN492941 [GenBank]
Sequencing primer gene specific primer, AB24: 5'-CACTAGACCTTTTTCTTTGGTTCG-3'
Sequence (A. th genome BLAST matches underlined) 
>15-K103785-0022-784-H03C-AB24
TGGATCATTTCGGCTTTGTGGATTGAATACGATGAACTAGCTATCCCACTCGGACTTCGT
ATGACCCAAAGAAACCGTTTTCCACTCTCCGCTAGGCCAAGAGCCAGCTCAATGAACTGC
TCAAACGTGAGTGTTCCGCCGCTTCCAAAGGATACGTATAGAACCGACCCGAATGGTTGG
TTGTCTAGCCAATTTAAACATTTGTACTCATCGTTCACGTCAGCATCGTGTGAACCCGAG
TTAACCAACGGCCCAATCAGGTAAACCGGTGGTTTATCAGGAGCCGGTTCTTGTACAATC
TTTATAGTGTTTGGCTCTAAATCGACGAAGGAATTCACTAGAATCCCTTCAGCTTCTTTG
AATCTCTTGACGTTGTGTAGAAGCCATTTGTATGATTCATCTTTTCGGTCTTGACACGGA
TCGACGAAATCCTTACCGGTTATGGGGACACAACCGGGAATAATAACCGGTTCGGTTAAT
TCCCTAAACTCACACGACACCGTTTCGTCTACATGTATATTCAATTGTAAATGGCTTCAT
GTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAG
AGTAATTACCAATTTTTTTTCAATTCANAAATGTAGATGTCCGCAGCGT
   
Sequence Name 31-K103786-0022-784-H03C-8409
GenBank Accession LN500132 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>31-K103786-0022-784-H03C-8409
GCCTTTACATTGAATATACATGTAGACGAAACGGTGTCGTGTGAGTTTAGGGAATTAACC
GAACCGGTTATTATTCCCGGTTGTGTCCCCATAACCGGTAAGGATTTCGTCGATCCGTGT
CAAGACCGAAAAGATGAATCATACAAATGGCTTCTACACAACGTCAAGAGATTCAAAGAA
GCTGAAGGGATTCTAGTGAATTCCTTCGTCGATTTAGAGCCAAACACTATAAAGATTGTA
CAAGAACCGGCTCCTGATAAACCACCGGTTTACCTGATTGGGCCGTTGGTTAACTCGGGT
TCACACGATGCTGACGTGAACGATGAGTACAAATGTTTAAATTGGCTAGACAACCAACCA
TTCGGGTCGGTTCTATACGTATCCTTTGGAAGCGGCGGAACACTCACGTTTGAGCAGTTC
ATTGAGCTGGCTCTTGGCCTAGCGGAGAGTGGAAAACGGTTTCTTTGGGTCATACGAAGT
CCGAGTGGGATAGCTAGTTCATCGTATTTCAATCCACAAAGCCGAAATGATCCATTTTCG
TTTTTACCACAAGGCTTCTTAGACCGAACCAAAGAAAAAGGTCTAGTG

Last Updated on 06.12.2018 16:11