Sequences from confirmation amplicon


Confirmation sequences for line 785B12 and hit At5g48630 are shown.
Line ID 785B12
Locus At5g48630 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:19722511
Primers used for PCR MI44 - 8474, show primer details
   
Sequence Name 64-K032734-022-785-B12C-MI44
GenBank Accession LN511126 [GenBank]
Sequencing primer gene specific primer, MI44: 5'-AACAACAGAGATAACAGTGCATGG-3'
Sequence (A. th genome BLAST matches underlined) 
>64-K032734-022-785-B12C-MI44
GCTCTTCAACCATGTCTTTATGTCTTTCTCTTTGTGTACAGAAGCAATGTAAATGCAAGC
GAGTGTTATGAGAAACGGCGGATGTATAAGGATGAGGTCCATTCTATATGTGTCGTTGAC
AAGACCCCTAAAGTTTCCACAACAAAACTTAAAGCATCAAGAGAGCATTTACTTTTTTGC
TTGCAAATCAAAACCTGTTTAGGCTCTCTTCCAAGAAGCTTAATGAAGGACAAAAGTTTT
GGACACTTACCAAGTTAGATGGGTCATACTAGTGTCATTGATTCCAGAATCCTGCAAGAA
CCTGCAAGTAATGCAAGAAGGAATAATCAAAACGAAATGCAGAGTATAATGGCAAAAAGA
TTTCGATGACGACACATATGATCCTAAGCAAACGACTTACTCAGGCAGAGATCGATATGG
GTGGAATACAACAAGATAGAAGTTCAATGCTTCCAAGACCTTCATCTCCATTTCCAGAAT
ATCTTTAATCTCATATCGAAACTTTTCATCCGCATCTACAGAGAGTGAAAGCATCCAATC
TCATCAGAAACTCAGACAAAAAAAACTCAAGAAAATTTTGGAAGCAGAAAACAAAAAAGA
ATTTACAAAGTTTTTTCATGTAGAAGACAAGAAGTTTGGCATGTCCGGGAAATCTACATG
GATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTT
TTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAA
AACGACAAATTACGATCCGTCGTATTTATAGG
   
Sequence Name 32-K032734-022-785-B12C-8409
GenBank Accession LN500474 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>32-K032734-022-785-B12C-8409
CGGAATGCAACTTCTGGCTTCTACATGAAAAAACTTTGTAAATTCTTTTTTGTTTTCTGC
TTCCAAAATTTTCTTGAGTTTTTTTTGTCTGAGTTTCTGATGAGATTGGATGCTTTCACT
CTCTGTAGATGCGGATGAAAAGTTTCGATATGAGATTAAAGATATTCTGGAAATGGAGAT
GAAGGTCTTGGAAGCATTGAACTTCTATCTTGTTGTATTCCACCCATATCGATCTCTGCC
TGAGTAAGTCGTTTGCTTAGGATCATATGTGTCGTCATCGAAATCTTTTTGCCATTATAC
TCTGCATTTCGTTTTGATTATTCCTTCTTGCATTACTTGCAGGTTCTTGCAGGATTCTGG
AATCAATGACACTAGTATGACCCATCTAACTTGGTAAGTGTCCAAAACTTTTGTCCTTCA
TTAAGCTTCTTGGAAGAGAGCCTAAACAGGTTTTGATTTGCAAGCAAAAAAGTAAATGCT
CTCTTGATGCTTTAAGTTTTGTTGTGGAAACTTTAGGGGTCTTGTCAACGACACATATAG
AATGGACCTCATCCTTATACATCCGCCGTTTCTCATAACACTCGCTTGCATTTACATTGC
TTCTGTACACAAAGAGAAAGACATAAAGACATGGTTTGAAGAGCTTTCTGTCGACATGAA
CATTGTAAGTCCATGCACTG

Last Updated on 06.12.2018 16:11