Sequences from confirmation amplicon


Confirmation sequences for line 785E02 and hit At4g31810 are shown.
Line ID 785E02
Locus At4g31810 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:15388848
Primers used for PCR 49kh - 8474, show primer details
   
Sequence Name 56-K113845-0022-785-E02C-49kh
GenBank Accession LN509167 [GenBank]
Sequencing primer gene specific primer, 49kh: 5'-CCAAGGAACGTATTTAAAACCAAA-3'
Sequence (A. th genome BLAST matches underlined) 
>56-K113845-0022-785-E02C-49kh
TTTCGTAGATAAAAGGCAAGTGTTTGAAATATTTTCGAAACTTTAATTCTTGCTCTTAAG
CCTCTTATACATCAAGAGTTTTCGTTTAACTGCAGATAGCAATAATGGATGGTGTGACCA
TGGGTTGTGGTGGTGGAATTTCACTTCCAGGGATGTTTCGTGTGGCTACAGATAAAACTG
TATGTGGCTCGCTTTATTATTACAAATCACTGATTTTTTACTTCGAGTGGACGCAGTAAT
AAAAGCAAAGTCAGTGACTACATAAGATCCTAGAGTCTCTTAAGAAAAGTAGTTAAATTT
CTTAGCCATTTTCTCTGTATTTTAAATATCTTGTTGCCTTAGTCAATTATCAGTAGCCCA
TTTCTCTTTGTCTGAAACTGTTTCTTTTCTTGTAAATGGCTTCATGTCCGGGAAATCTAC
ATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTT
TTTTTCAATTCAAAAATGTA
   
Sequence Name 29-K113844-0022-785-E02C-8409
GenBank Accession LN499376 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>29-K113844-0022-785-E02C-8409
GTTTTCAGACAAAGAGAAATGGGCTACTGATAATTGACTAAGGCAACAAGATATTTAAAA
TACAGAGAAAATGGCTAAGAAATTTAACTACTTTTCTTAAGAGACTCTAGGATCTTATGT
AGTCACTGACTTTGCTTTTATTACTGCGTCCACTCGAAGTAAAAAATCAGTGATTTGTAA
TAATAAAGCGAGCCACATACAGTTTTATCTGTAGCCACACGAAACATCCCTGGAAGTGAA
ATTCCACCACCACAACCCATGGTCACACCATCCATTATTGCTATCTGCAGTTAAACAAAA
CTCTTGATGTATAAGAGGCTTAAGAGCAAGAATTAAAGTTCAAAATATTTCAAACACTTG
CCTTTTATCTACAAAATCAGTTTCATAGATTATCGAGTGGACACAAATAAGTAGGTTAGC
TCTTTCTAGTCACACAAGGTCATACGAACCAATCCCATCTGGTATTAACAAATAGCATCG
AAAAAAATGGCTAGGAATCTTACATTTGGTTTT

Last Updated on 06.12.2018 16:11