Sequences from confirmation amplicon


Confirmation sequences for line 785G10 and hit At3g25610 are shown.
Line ID 785G10
Locus At3g25610 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:9311470
Primers used for PCR CN16 - 8474, show primer details
   
Sequence Name 37-K030758-022-785-G10C-8409
GenBank Accession LN502502 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>37-K030758-022-785-G10C-8409
GCCATTTACAATTGAATATTCATTATTGCGGTAGAGTTCGGGTTCGAGTTCTTTAACCGG
ACACAAAATGGGATCTCTTTTCGTGAACTGGATCTTGTATCGGGAGAGAAAGTTGAAAGG
TTCGTTTTTAGCCTTCTTTTCAATCAGATGTAGTCTCTGTGATTCCCGGAACTCAAAACC
TCCTCTGTTCTTGCAGGGTTTATAGGTTACTAAACGTTCTTGAATTCAATAGCACTAGGA
AGAGAATGTCTGTTATTGTAAGAGACGATGACGGGAAGCTCTTGCTGTTGTCTAAGGGAG
CTGATAAGTAAGCGAGTTTAGACATTTTTTAGACATTTTGGTTCATTATAAAATCTAACT
TTGAGTTTGGTGTTCTCTGTGTAGTGTAATGTTCGAAAGACTTGCA
   
Sequence Name 76-K030758-022-785-G10C-CN16
GenBank Accession LN513322 [GenBank]
Sequencing primer gene specific primer, CN16: 5'-TTGCAAGTCTTTCGAACATTACAC-3'
Sequence (A. th genome BLAST matches underlined) 
>76-K030758-022-785-G10C-CN16
ACTCAAGTTAGATTTTATAATGAACCAAAATGTCTAAAAAATGTCTAAACTCGCTTACTT
ATCAGCTCCCTTAGACAACAGCAAGAGCTTCCCGTCATCGTCTCTTACAATAACAGACAT
TCTCTTCCTAGTGCTATTGAATTCAAGAACGTTTAGTAACCTATAAACCCTGCAAGAACA
GAGGAGGTTTTGAGTTCCGGGAATCACAGAGACTACATCTGATTGAAAAGAAGGCTAAAA
ACGAACCTTTCAACTTTCTCTCCCGATACAAGATCCAGTTCACGAAAAGAGATCCCATTT
TGTGTCCGGTTAAAGAACTCGAACCCGAACTCTACCGCAATAATGAATATTCAATTGTAA
ATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAG
AAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTT
ATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGG
CGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTACATTCA
CTCCCAAAATGG

Last Updated on 06.12.2018 16:11