Sequences from confirmation amplicon


Confirmation sequences for line 786B08 and hit At5g57170 are shown.
Line ID 786B08
Locus At5g57170 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:23162337
Primers used for PCR 95rq - 8474, show primer details
   
Sequence Name 67-K116204-0022-786-B08C-95rq
GenBank Accession LN511889 [GenBank]
Sequencing primer gene specific primer, 95rq: 5'-CAAAGTGGATTTATGGGCTACAAT-3'
Sequence (A. th genome BLAST matches underlined) 
>67-K116204-0022-786-B08C-95rq
ACAAATTATGAGATCATATCACACAAGTGACAAACTAGGCCCAAAAGCAGTTTAAATACG
AAATATATTGGCTTTGAGGGAGGTAAAATTATAAATTATGGTTCAGAATTACAGCTTTTT
GAAATGAATAAGCAAACAGTTCTAACCCAAATATCATTACAAGGTTGAATTCGCCTCTTT
TCAGAAAGTTGATCCATTGTAACCGAAGAAAGGTCGCTGCCAAAGAAAAGCAAAACAAAG
AATTTAACTAACATTCTATTCATTGATCAAGTTTATAAGAACTAAAGAACATCGTGCTTT
CATCTAAGTGTGTATCCCATCATCATGGCTTCAATTGTAAATGGCTTCATGTCCGGGAAA
TCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACC
AATTTTTTTTCAATTCAAAAATGTAGATGTCC
   
Sequence Name 31-K116203-0022-786-B08C-8409
GenBank Accession LN500302 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>31-K116203-0022-786-B08C-8409
GGAAGAACCTTTAAATTGAGCCATGATGATGGGATACACACTTAGATGAAAGCACGATGT
TCTTTAGTTCTTATAAACTTGATCAATGAATAGAATGTTAGTTAAATTCTTTGTTTTGCT
TTTCTTTGGCAGCGACCTTTCTTCGGTTACAATGGATCAACTTTCTGAAAAGAGGCGAAT
TCAACCTTGTAATGATATTTGGGTTAGAACTGTTTGCTTATTCATTTCAAAAGCTGTAAT
TCTGAACCATAATTTATAATTTTACCTCCCTCAAAGCCAATATATTTCGTATTTAAACTG
CTTTTGGGCCTAGTTTGTCACTTGTGTGATATGATCTCATAATTTTGTCAATACTACTAA
AAATGTAAACTGCAGCCCAAAAAAAAATTGTAGCCCATAATCCCACTTTG

Last Updated on 06.12.2018 16:11