Sequences from confirmation amplicon


Confirmation sequences for line 786C10 and hit At5g08030 are shown.
Line ID 786C10
Locus At5g08030 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:2575443
Primers used for PCR 32ai - 8474, show primer details
   
Sequence Name 36-K106635-0022-786-C10C-8409
GenBank Accession LN502237 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>36-K106635-0022-786-C10C-8409
GCCATTTACTCATTCGCGTAATCTTCAGGTTTGTGTGTAGGACTTGAACTCCTGTCTCCA
CTCTCTTACTATCATCACTGATTTGTTTCATTAAACTTCACGTTGAATAATGTTTTTGCT
AGGTCCATCCATACACATACCGTAATGAGAACCAGTTTCTGCATTTAGAGTTTAATCAGG
ACCCTTACCTAGAGTATGATTATTGGCTCAATAAGATCGGTGTTGATGGTTTGTTCACTG
ATTTCACTGGAAGTCTACACAATTACCAGGAATTGAAGTCTCCTTTGCCTCAGCAACAGT
GATTCTCCAATTGAATTTGTTCTTTTCTTTCTTATGCTAGTTTATGTTCTCTTAGGATTT
TTTTTTACAAACCCATGGATGTTTCCATTTTCTTCGATTTCATAATATATTGCTTCTACT
TTTTCCAAATGGCTCTCTGAATTGATTGTTGGAACA
   
Sequence Name 52-K106636-0022-786-C10C-32ai
GenBank Accession LN507830 [GenBank]
Sequencing primer gene specific primer, 32ai: 5'-TGTTCCAACAATCAATTCAGAGAG-3'
Sequence (A. th genome BLAST matches underlined) 
>52-K106636-0022-786-C10C-32ai
GCATAATTATGAATCGAAGAAATGGAAACATCCATGGGTTTGTAAAAAAAAATCCCAATA
GAACATAAACTAGCATAAGAAAGAAAAGAACAAATTCAATTGGAGAATCACTGTTGCTGA
GGCAAAGGAGACTTCAATTCCTGGTAATTGTGTAGACTTCCAGTGAAATCAGTGAACAAA
CCATCAACACCGATCTTATTGAGCCAATAATCATACTCTAGGTAAGGGTCCTGATTAAAC
TCTAAATGCAGAAACTGGTTCTCATTACGGTATGTGTATGGATGGACCTAGCAAAAACAT
TATTCAACGTGAAGTTTAATGAAACAAATCAGTGATGATAGTAAGAGAGTGGAGACAGGA
GTTCAAGTCCTACACACAAACCTGAAGATTACGCGAATGAGTAAATGGCTTCATGTCCGG
GAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAAT
TACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCACGNTTATT

Last Updated on 06.12.2018 16:11