Sequences from confirmation amplicon


Confirmation sequences for line 787A06 and hit At3g55880 are shown.
Line ID 787A06
Locus At3g55880 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:20737878
Primers used for PCR 32ve - 8474, show primer details
   
Sequence Name 26-K117967-0022-787-A06C-32ve
GenBank Accession LN498119 [GenBank]
Sequencing primer gene specific primer, 32ve: 5'-GAGACGAAAGCTCCTAGTCTTCAA-3'
Sequence (A. th genome BLAST matches underlined) 
>26-K117967-0022-787-A06C-32ve
TTTATTAACTTTTAGCCGTTACATAAAGCATTATTAGCATTAAAAAAATGATATAGGCCG
TATCAAAATTGTTTATTTTTCAAATCTAGCATTAGTTATTTGTTTTTATATCTTAAGATA
TTGTTCTTCTACCACTAATACAGATCAGACTCGGTCAGAAATAGCATGTGACTTCAGATA
TTACATTATAGTCACCGTCTTGAATTAATTTTCACATTTTTTTTGGTACCAAGTGTTATT
GTAGGTTTGAAAATGACTAGTCAATTGAGGAAATTCAATTGTAAATGGCTTCATGTCCGG
GAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAAT
TACCAATTTTTTTTCAATTCAAAAATGTAGATGTCC
   
Sequence Name 10-K117966-0022-787-A06C-8409
GenBank Accession LN490855 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>10-K117966-0022-787-A06C-8409
ATTTAAATTGAATTTCCTCAATTGACTAGTCATTTTCAAACCTACAATAACACTTGGTAC
CAAAAAAAATGTGAAAATTAATTCAAGACGGTGACTATAATGTAATATCTGAAGTCACAT
GCTATTTCTGACCGAGTCTGATCTGTATTAGTGGTAGAAGAACAATATCTTAAGATATAA
AAACAAATAACTAATGCTAGATTTGAAAAATAAACAATTTTGATACGGCCTATATCATTT
TTTTAATGCTAATAATGCTTTATGTAACGGCTAAAAGTTAATAAAATAAGAAAAATCAAA
GTGAACTTACGGATTCGATGTCGATTTTGAAGACTAGGACTTTTTCGTC

Last Updated on 06.12.2018 16:11