Sequences from confirmation amplicon


Confirmation sequences for line 787B04 and hit At2g23410 are shown.
Line ID 787B04
Locus At2g23410 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:9966694
Primers used for PCR 25gz - 8474, show primer details
   
Sequence Name 64-K112309-0022-787-B04C-25gz
GenBank Accession LN511234 [GenBank]
Sequencing primer gene specific primer, 25gz: 5'-TACAATGATACGCAAGAAGTTTGG-3'
Sequence (A. th genome BLAST matches underlined) 
>64-K112309-0022-787-B04C-25gz
ATGAAGGTGGATCAACCCGACACCAAATCGTCTTTCCCTACGCTGATATGAAGCAAGAGC
CTCTAGAAGCTTATCCTTATCGAAATCAGGCCAAAATACCGGCGAGAAGAAAAGCTCGGA
ATAAGCAAGTTGCCACAAGAAGAAGTTACTAATCCTCTGTTCTCCACTTGTCCTAATCAT
TAAATCAGGACTTGGGAAGTCACTACAATTTGTCAAAAGCTCTCTCTCGATCAATGCCTC
GTCTACATCTTCCTCTCGGATCAACCCTTTTTCTGATTTCTTCACAAGACTCTTGCAAGC
ATGCATGATATCAAATTTCCCGCTGTAATCTACTGCCATGATGAGATGTTTATTCTTGTA
GCCTTTGGTAGCTTCCTCTATCTCATGGATTTCTTTGAGGAGAGATTCAGGGATCTTCGT
TTTATTCCCGATAACAGAAACGCGAACTTCCGATCTGTGGAAAAATGGCTTCATGTCCGG
GAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAAT
TACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCA
   
Sequence Name 48-K112308-0022-787-B04C-8409
GenBank Accession LN506539 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>48-K112308-0022-787-B04C-8409
CATTTTTCCCAGATCGGAAGTTCGCGTTTCTGTTATCGGGAATAAAACGAAGATCCCTGA
ATCTCTCCTCAAAGAAATCCATGAGATAGAGGAAGCTACCAAAGGCTACAAGAATAAACA
TCTCATCATGGCAGTAGATTACAGCGGGAAATTTGATATCATGCATGCTTGCAAGAGTCT
TGTGAAGAAATCAGAAAAAGGGTTGATCCGAGAGGAAGATGTAGACGAGGCATTGATCGA
GAGAGAGCTTTTGACAAATTGTAGTGACTTCCCAAGTCCTGATTTAATGATTAGGACAAG
TGGAGAACAGAGGATTAGTAACTTCTTCTTGTGGCAACTTGCTTATTCCGAGCTTTTCTT
CTCGCCGGTATTTTGGCCTGATTTCGATAAGGATAAGCTTCTAGAGGCTCTTGCTTCGTA
TCAGCGTAGGGAAAGACGATTTGGTTGTCGGGTTTGATCCACCTTTCATCCTCTCTATNA
TGTTTTGTTCATCTGTAATTTCTACCAAACTTCTTG

Last Updated on 06.12.2018 16:11