Sequences from confirmation amplicon


Confirmation sequences for line 787E05 and hit At4g31140 are shown.
Line ID 787E05
Locus At4g31140 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:15142544
Primers used for PCR 39qz - 8474, show primer details
   
Sequence Name 79-K115869-0022-787-E05C-39qz
GenBank Accession LN513867 [GenBank]
Sequencing primer gene specific primer, 39qz: 5'-TGAGGATCCTGAAGATTAGCATTT-3'
Sequence (A. th genome BLAST matches underlined) 
>79-K115869-0022-787-E05C-39qz
TATGAACATCCTTTGCAGGGATCAGCCCGTTACCACTGCCAAGACTGAGCTGATACTTAG
GCTGTCCATCAATATAAAATATCCCCCAGTGTCTTTCAAAGTTTCCAGGCTGAATGCTTT
TGGCATCTTCATCGATAAGACCAAACAAGTAAGCATCCATTGCCCCAGGTCTCATAGGTG
TTCCTTTATTAGCCTTTTGACGGTTCATAAAACCTTGATTATACCGCCTGGCATACATCA
AGTTTGCGTTCTTATCTCCATCTGTTGGATCAGCAATGTAAATGGCTTCATGTCCGGGAA
ATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTAC
CAATTTTTTTTCAATTCAAAAATGTAGATGTCCGC
   
Sequence Name 38-K115867-0022-787-E05C-8409
GenBank Accession LN503105 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>38-K115867-0022-787-E05C-8409
CTGATCCAACAGATGGAGATAAGAACGCAAACTTGATGTATGCCAGGCGGTATAATCAAG
GTTTTATGAACCGTCAAAAGGCTAATAAAGGAACACCTATGAGACCTGGGGCAATGGATG
CTTACTTGTTTGGTCTTATCGATGAAGATGCCAAAAGCATTCAGCCTGGAAACTTTGAAA
GACACTGGGGGATATTTTATATTGATGGACAGCCTAAGTATCAGCTCAGTCTTGGCAGTG
GTAACGGGCTGATCCCTGCAAAGGATGTTCATTATTTGGCTAAGAAATGGTGTATTCTTG
CGCCAAATGCTAATCTCANGGATCCTC

Last Updated on 06.12.2018 16:11