Sequences from confirmation amplicon


Confirmation sequences for line 787F04 and hit At1g61740 are shown.
Line ID 787F04
Locus At1g61740 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:22800809
Primers used for PCR DA03 - 8474, show primer details
   
Sequence Name 13-K105749w-0022-787-F04C-DA03
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, DA03: 5'-GCGAAGTAAGAAAGCCAAATAATG-3'
Sequence (A. th genome BLAST matches underlined) 
>13-K105749w-0022-787-F04C-DA03
AACCTCTTTGGTCTCTGATAATCCTCCAAGAGAGGCAACTTTAGACTCTCAATCTTATCT
TCTTCATCATCTTCTTCAATTCTATTCGATTCTCTAATCTTCACCATCTCCGATTCCAAT
CTCCAATAATAAAGTCCATTTCCAAAAGTCTTCAACGTCGACCACGCAAGAAACACAGCG
AAGAGACTCGTAATCAACCAGTTCGGAAAAACAAGATTACAGATCACACCAATACTAACT
CCAAGAAGCATACATGGTTCCAATAAAAGAGCTAAATCGAAGTCGATTAGGGTTTTGCCA
CCAGATTTAGGGTTTCTAACGAATAGATTGCATCCCACGTTAGCTATAGATCCACCAGTA
ACCATAAAAGCTGAGAAGCTTGAAGCTGTCTTTAGATCGAGACCGGCCACGATTGTCATT
ATCGGAACATATAAACCTCCACCGCCGATTCCACCGGCGCTTGAGATTGTAAATGGCTTC
ATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAA
AGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCC
   
Sequence Name 53-K105750-0022-787-F04C-8409
GenBank Accession LN508156 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>53-K105750-0022-787-F04C-8409
GCCATTTACAATCTCAAGCGCCGGTGGAATCGGCGGTGGAGGTTTATATGTTCCGATAAT
GACAATCGTGGCCGGTCTCGATCTAAAGACAGCTTCAAGCTTCTCAGCTTTTATGGTTAC
TGGTGGATCTATAGCTAACGTGGGATGCAATCTATTCGTTAGAAACCCTAAATCTGGTGG
CAAAACCCTAATCGACTTCGATTTAGCTCTTTTATTGGAACCATGTATGCTTCTTGGAGT
TAGTATTGGTGTGATCTGTAATCTTGTTTTTCCGAACTGGTTGATTACGAGTCTCTTCGC
TGTGTTTCTTGCGTGGTCGACGTTGAAGACTTTTGGAAATGGACTTTATTATTGGAGATT
GGAATCGGAGATGGTGAAGATTAGAGAATCGAATAGAATTGAAGAAGATGATGAAGAAGA
TAAGATTGAGAGTCTAAAGTTGCCTCTCTTGGAGGATTATCAGAGACCAAAGAGGTTTCC
ATGGATTAAGCTTGGAGTTTTGGTCATTATTTGGCTTCTTTACTTCGC

Last Updated on 06.12.2018 16:11