Sequences from confirmation amplicon


Confirmation sequences for line 789E07 and hit At1g07030 are shown.
Line ID 789E07
Locus At1g07030 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:2159571
Primers used for PCR 32fp - 8474, show primer details
   
Sequence Name 83-K111396-0022-789-E07C-32fp
GenBank Accession LN514329 [GenBank]
Sequencing primer gene specific primer, 32fp: 5'-TATATCAAGCGATGCTGTTTTCAC-3'
Sequence (A. th genome BLAST matches underlined) 
>83-K111396-0022-789-E07C-32fp
TGGGTGAGGGACTTATAAGGAGTCTGGGATTGTGTTAAGAGGGTTTTGCGTGAGGAAGGG
ATTGGTGCGTTTTATGCTTCTTATAGGACGACGGTTCTGATGAACGCGCCGTTTACGGCT
GTTCACTTTGCCACTTACGAGGCTGCTAAGAAGGGATTGATGGAGTTTTCGCCGGATAGA
ATTAGTGATGAGGAAGGTTGGTTGGTTCACGCTACCGCTGGAGCTGCTGCTGGTGGATTG
GCAGCTGCTGTGACTACGCCGCTTGATGTTGTCAAGACGCAGTTGCAATGTCAGGTGAAT
GATTCCTTCTCTCTAGGATTGGTTGCTCTTTGTCTCTAATCTCTACATCTTTGGCTAGAA
TAATGTTGGCGATGTGATTAGGTACACAGTGGTGTTAGTGTTACTAGTAAACGGTATATG
TGTTATGCTTTTATGTGTGCGTAGTGCTGCAGAATGCTTCTTTAAATGATGCTTCTTTCA
ATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAA
TATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCG
CAGCGTTATTA
   
Sequence Name 76-K111395-0022-789-E07C-8409
GenBank Accession LN513421 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>76-K111395-0022-789-E07C-8409
AGAAGCATCATTTAAAGAAGCATTCTGCAGCACTACGCACACATAAAAGCATAACACATA
TACCGTTTACTAGTAACACTAACACCACTGTGTACCTAATCACATCGCCAACATTATTCT
AGCCAAAGATGTAGAGATTAGAGACAAAGAGCAACCAATCCTAGAGAGAAGGAATCATTC
ACCTGACATTGCAACTGCGTCTTGACAACATCAAGCGGCGTAGTCACAGCAGCTGCCAAT
CCACCAGCAGCAGCTCCAGCGGTAGCGTGAACCAACCAACCTTCCTCATCACTAATTCTA
TCCGGCGAAAACTCCATCAATCCCTTCTTAGCAGCCTCGTAAGTGGCAAAGTGAACAGCC
GTAAACGGCGCGTTCATCAGAACCGTCGTCCTATAAGAAGCATAAAACGCACCAATCCCT
TCCTCACGCAAAACCCTCTTAACACAATCCCAGACTCCTTTATAAGTCCCTTCACCCATC
TGCAACCTCTGCTTCACCATATCCATGGGAGTGAAAACAGCATCGCTTGATAT

Last Updated on 06.12.2018 16:11