Sequences from confirmation amplicon


Confirmation sequences for line 789H12 and hit At4g32410 are shown.
Line ID 789H12
Locus At4g32410 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:15641839
Primers used for PCR 28my - 8474, show primer details
   
Sequence Name 40-K114393-0022-789-H12C-28my
GenBank Accession LN503791 [GenBank]
Sequencing primer gene specific primer, 28my: 5'-TGAAGGTTTGATTGAGCTGAT-3'
Sequence (A. th genome BLAST matches underlined) 
>40-K114393-0022-789-H12C-28my
TGATGGTGAATCTTAAATCCTTGTTTATGCAGGTTATGATGATGAGAGGTCTATTCTAAT
GTCCCAGAGGAGTGTAGAGAAGCGTTTTGGTCAGTCGCCGGTATTTATTGCGGCAACCTT
CATGGAACAAGGCGGCATTCCACCAACAACCAATCCCGCTACTCTTCTGAAGGAGGCTAT
TCATGTTATAAGCTGTGGTTACGAAGACAAGACTGAATGGGGCAAAGAGGTCAGTTTTCA
AATGCAGCTACAGAATCTTCTTATGTTCTCTTTCTTACCTGTTTGATGACATCTTATTTG
GCACTTTTGTTAGATTGGTTGGATCTATGGTTCCGTGACGGAAGATATTCTTACTGGGTT
CAAGATGCATGCCCGGGGTTGGATATCGATCTACTGCAATCCTCCACGCCCTGCGTTCAA
GGGATCTGCACCAATCAATCTTTCTGATCGTTTGAACCAAGTTCTTCGATGGGCTTTGGG
ATCTATCGAGATTCTTCTTAGCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAA
ATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTAC
CAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAC
   
Sequence Name 16-K114392-0022-789-H12C-8409
GenBank Accession LN493523 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>16-K114392-0022-789-H12C-8409
ATTTACATTGAATATATCCTGCTAAGAAGAATCTCGATAGATCCCAAAGCCCATCGAAGA
ACTTGGTTCAAACGATCAGAAAGATTGATTGGTGCAGATCCCTTGAACGCAGGGCGTGGA
GGATTGCAGTAGATCGATATCCAACCCCGGGCATGCATCTTGAACCCAGTAAGAATATCT
TCCGTCACGGAACCATAGATCCAACCAATCTAACAAAAGTGCCAAATAAGATGTCATCAA
ACAGGTAAGAAAGAGAACATAAGAAGATTCTGTAGCTGCATTTGAAAACTGACCTCTTTG
CCCCATTCAGTCTTGTCTTCGTAACCACAGCTTATAACATGAATAGCCTCCTTCAGAAGA
GTAGCGGGATTGGTTGTTGGTGGAATGCCGCCTTGTTCCATGAAGGTTGCCGCAATAAAT
ACCGGCGACTGACCAAAACGCTTCTCTACACTCCTCTGGGACATTAGAATAGACCTCTCA
TCATCATAACCTGCATAAACAAGGATTGTAAGATTTCACCATCACATAAATCATTACATA
AAGAAGTGATGTTATTACACAATCAGCTCAATCAAAACCTTC

Last Updated on 06.12.2018 16:11