Sequences from confirmation amplicon


Confirmation sequences for line 790D04 and hit At4g00970 are shown.
Line ID 790D04
Locus At4g00970 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:420877
Primers used for PCR BA19 - 8474, show primer details
   
Sequence Name 79-K104767-0022-790-D04C-BA19
GenBank Accession LN513826 [GenBank]
Sequencing primer gene specific primer, BA19: 5'-CAATTACAACCCCAAACTCAAAGT-3'
Sequence (A. th genome BLAST matches underlined) 
>79-K104767-0022-790-D04C-BA19
GCAGAGTTTATTCCTTACATAGCTAAGGAGGAATAATGAGCTGTCTTCTTCTGGAGACCA
ATTGTTTTTCTTACCTTTAATAATCTCAAGGACTAGTACACCGAAGCTAAATACATCGGT
TTTTACCGAGAATTCGCCACTCATTGCATACTCTGGAGCCATGTAACCGCTGCCAAACGA
GCAAATAATGTTTTGGCTTTCATTTTAGTTTTACAGAATTGATAATAACTTAAAGGGTAG
TTTTTAAAATGGTAGCTTACTAGGTTCCTGCAACTTTGCTTGTAAACCTGGTCTGACTTG
TTTGGTCAGTATCAAACAACTTTGCCATTCCAAAATCCGCGATTTTCGGATTCATTGCAT
CGTCCAATAGAACATTGCTGGCTTTCATATCTCTATGAACAATCTTGATATGGAGAAAAA
GAAAGAGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGT
CAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGT
CCGCACGATTATT
   
Sequence Name 79-K104768-0022-790-D04C-OriL
GenBank Accession LN513827 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>79-K104768-0022-790-D04C-OriL
TCTTTCTTTTTCTCCATATCAAGATTGTTCATAGAGATATGAAAGCCAGCAATGTTCTAT
TGGACGATGCAATGAATCCGAAAATCGCGGATTTTGGAATGGCAAAGTTGTTTGATACTG
ACCAAACAAGTCAGACCAGGTTTACAAGCAAAGTTGCAGGAACCTAGTAAGCTACCATTT
TAAAAACTACCCTTTAAGTTATTATCAATTCTGTAAAACTAAAATGAAAGCCAAAACATT
ATTTGCTCGTTTGGCAGCGGTTACATGGCTCCAGAGTATGCAATGAGTGGCGAATTCTCG
GTAAAAACCGATGTATTTAGCTTCGGTGTACTAGTCCTTGAGATTATTAAAGGTAAGAAA
AACAATTGGTCTCCAGAAGAAGACAGCTCATTATTCCTCCTTAGCTATGTAAGTGAATAA
AACTCTTGCTTTGTTTTTTTTGACTCTAGACTTTGAGTTTGGGGTTGTAATTG
Primers used for PCR BA18 - 8474, show primer details
   
Sequence Name 78-K104767-0022-790-D04C-BA18
GenBank Accession LN513688 [GenBank]
Sequencing primer gene specific primer, BA18: 5'-ATGAGATGAGTAGTTCATGTTGCG-3'
Sequence (A. th genome BLAST matches underlined) 
>78-K104767-0022-790-D04C-BA18
TTTGTACCAAGCAAGTAATCGCCATCATCATCATCATCAAAAAGAGACATCACCTTAATT
TTGAAACCAAGCACTCCAAAGACTTGATCTCTTTGACATGTGTTTAAATAATCACCAGGG
TGTTCTTGATTATGGGGAAGAGATAGCTGTGAAAAGATTGTCAATGAAATCAGGACAAGG
AGACAATGAGTTCATAAACGAAGTCTCATTGGTTGCAAAACTCCAACATCGTAACCTTGT
TAGGCTCTTAGGTTTCTGCTTACAAGGAGAAGAACGAATTCTCATCTATGAGTTCTTCAA
GAACACAAGCCTAGACCATTACATATTTGGTACCTTAAACTAAACCCCAAACAAATGTAT
CTCTCTATTTATAGTAACTTATTTAATGATTGATCCAAATTAGTTGTTTTCTTTCGCAGA
TTCTAATAGGAGAATGATCTTAGATTGGGAAACACGTTATAGGATTATCTCAGGTGTTGC
TAGAGGTCTTCTCTATCTCCATGAAGATTCTCGTTTCAATTGTAAATGGCTTCATGTCCG
GGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAA
TTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCG
   
Sequence Name 78-K104768-0022-790-D04C-8409
GenBank Accession LN513689 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>78-K104768-0022-790-D04C-8409
GCCATTTACATTGAAACGAGAATCTTCATGGAGATAGAGAAGACCTCTAGCAACACCTGA
GATAATCCTATAACGTGTTTCCCAATCTAAGATCATTCTCCTATTAGAATCTGCGAAAGA
AAACAACTAATTTGGATCAATCATTAAATAAGTTACTATAAATAGAGAGATACATTTGTT
TGGGGTTTAGTTTAAGGTACCAAATATGTAATGGTCTAGGCTTGTGTTCTTGAAGAACTC
ATAGATGAGAATTCGTTCTTCTCCTTGTAAGCAGAAACCTAAGAGCCTAACAAGGTTACG
ATGTTGGAGTTTTGCAACCAATGAGACTTCGTTTATGAACTCATTGTCTCCTTGTCCTGA
TTTCATTGACAATCTTTTCACAGCTATCTCTTCCCCATAATCAAGAACACCCTGGTGATT
ATTTAAACACATGTCAAAGAGATCAAGTCTTTGGAGTGCTTGGTTTCAAAATTAAGGTGA
TGTCTCTTTTTGATGATGATGATGATGGCGATTACTTTGCTTGGTTACAAACCTTCATCT
CATGTCATAAGAATAAACCGCAACATGAACTACTCATCTCA

Last Updated on 06.12.2018 16:11