Sequences from confirmation amplicon


Confirmation sequences for line 790F11 and hit At2g19930 are shown.
Line ID 790F11
Locus At2g19930 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:8610053
Primers used for PCR HP53 - 8474, show primer details
   
Sequence Name 87-K033555-022-790-F11C-8409
GenBank Accession LN514713 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>87-K033555-022-790-F11C-8409
TTACAATTGAATAATCCTGCCGCCGCTGCCGCTTTGCATCAAGCATAAATAGGGATATGA
ACTATATAGTATACATACCGGNGTCTTGGTAACATGCTTCCTGTCCAACAAAATATGAAA
GCTTACTCTAAATTTAAATCAAGTATGAAGAAGGAAAAAAGATGATACGAGTGAACAAAG
GGCCTAGTCAACCATCAACGTCAGAGTAAGGACCTGAATACTTTCACTTCTGAGACATTT
CCCTTTAAATATATTTAGGGGACACATCCGAGCCAGGTCCTCAGAGATGTAGCCAGTTCC
ATCTGAATGTATGCAAGGTTTTTTGTTCTTGTCAAGAACATCTTTCCCATCTTGATCCTG
CAATTGGAAGAAATGGAAAAAGGTCAAAGAACATAGCGAATGCAAGTAAGTAAAGGCATT
AGAGACGAAGTTCCTCAAGGAAACATGAATTAAGGACGTACATGGCAGTGTATATCATCA
ATTTGATCAAAGGTAATCCCAGTCAATGTCAA
   
Sequence Name 87-K033555-022-790-F11C-HP53
GenBank Accession LN514714 [GenBank]
Sequencing primer gene specific primer, HP53: 5'-GTTGACATGACTGGGATTACCTTT-3'
Sequence (A. th genome BLAST matches underlined) 
>87-K033555-022-790-F11C-HP53
ACGTCCTTAATTCATGTTTCCTTGAGGAACTTCGTCTCTAATGCCTTTACTTACTTGCAT
TCGCTATGTTCTTTGACCTTTTTCCATTTCTTCCAATTGCAGGATCAAGATGGGAAAGAT
GTTCTTGACAAGAACAAAAAACCTTGCATACATTCAGATGGAACTGGCTACATCTCTGAG
GACCTGGCTCGGATGTGTCCCCTAAATATATTTAAAGGGAAATGTCTCAGAAGTGAAAGT
ATTCAGGTCCTTACTCTGACGTTGATGGTTGACTAGGCCCTTTGTTCACTCGTATCATCT
TTTTTCCTTCTTCATACTTGATTTAAATTTAGAGTAAGCTTTCATATTTTGTTGGACAGG
AAGCATGTTACCAAGACCCGGTATGTATACTATATAGTTCATATCCCTATTTATGCTTGA
TGCAAAGCGGCAGCGGCGGCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAAT
CTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCA
ATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATT
TTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTAT
TCTAATTCGGAAATCTTTATTTCGACGTGTCTACATTCA
Primers used for PCR 63wl - 8474, show primer details
   
Sequence Name 68-K118414-0022-790-F11C-63wl
GenBank Accession LN512097 [GenBank]
Sequencing primer gene specific primer, 63wl: 5'-ATAGCTCTAGGCACCAGAAACAAA-3'
Sequence (A. th genome BLAST matches underlined) 
>68-K118414-0022-790-F11C-63wl
ATTTTAGCACCATTAAGTGCAGCACGTTTGTTGTAGAATATGCTTTTAGACTCTTCCAAC
GNTGTTGAGCAATATATCCAAAAAGAATTCATTAGGGATTCCTCCATAGCTAAGCAGCGC
AACCAAATTTTTTGATAGCTTTGTTCTTTTTGGTGGATTACTGGATGATGGAAACAACTA
GATGTTACTAGGATAACTTCACTCCACAATAAGAACATTTGAGGGAGAGAACTTCAGTAT
TAAAACAACAAGTAGTCAGCGAACAAAATACCTTGTGGTTACTACTTCCAAGGCGTTAAA
GGTGCTAAAATTGGACAAGGATGGATCTTTAGACACCTTGATCATCGAAGGTCGAACCTG
GACAGTCCGAGGACAAAGCTGCCAAATTGGAAAGAAAATTAAGAATAGTTTTGCAAACAT
TTAAACCCCAAAAAAGGATGTTGTAATGTGAAAGAGAGCGGACTTACTTTCTTGTTTAAG
AGAAAAGTTCCCTTAACAGCATAACCATCATAAAACATCCGAAACTGGATCAAAAGAGGC
TGTTGGAAAAAGTAATATTTTACTACTTGAGTTCAATTGTAAATGGCTTCATGTCCGGGA
AATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTA
CCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCA
   
Sequence Name 20-K118413-0022-790-F11C-8409
GenBank Accession LN495420 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>20-K118413-0022-790-F11C-8409
TTGAACTCAAGTAGTAAAATATTACTTTTTCCAACAGCCTCTTTTGATCCAGTTTCGGAT
GTTTTATGATGGTTATGCTGTTAAGGGAACTTTTCTCTTAAACAAGAAAGTAAGTCCGCT
CTCTTTCACATTACAACATCCTTTTTTGGGGTTTAAATGTTTGCAAAACTATTCTTAATT
TTCTTTCCAATTTGGCAGCTTTGTCCTCGGACTGTCCAGGTTCGACCTTCGATGATCAAG
GTGTCTAAAGATCCATCCTTGTCCAATTTTAGCACCTTTAACGCCTTGGAAGTAGTAACC
ACAAGGTATTTTGTTCGCTGACTACTTGTTGTTTTAATACTGAAGTTCTCTCCCTCAAAT
GTTCTTATTGTGGAGTGAAGTTATCCTAGTAACATCTAGTTGTTTCCATCATCCAGTAAT
CCACCAAAAAGAACAAAGCTATCAAAAAATTTGGTTGCGCTGCTTAGCTATGGAGGAATC
CCTAATGAATTCTTTTTGGATATATTGCTCAACACGTTGGAAGAGTCTAAAAGCATATTC
TACAACAAACGTGCTGCACTTAATGGTGCTTAAAATTCCTAACGTTTCTGGTTCTTTTGC
ATTTTGTTTCTGGTGCCAAAAAGCTAT

Last Updated on 06.12.2018 16:11