Sequences from confirmation amplicon


Confirmation sequences for line 790G08 and hit At4g20110 are shown.
Line ID 790G08
Locus At4g20110 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:10875979
Primers used for PCR DA06 - 8474, show primer details
   
Sequence Name 35-K107369-0022-790-G08C-DA06
GenBank Accession LN501856 [GenBank]
Sequencing primer gene specific primer, DA06: 5'-AAACTCTTCTTGGTGGATTCTTCA-3'
Sequence (A. th genome BLAST matches underlined) 
>35-K107369-0022-790-G08C-DA06
CTTTCTCCTCGCGGCGTTGACCATCATCGCTATGGTCGTCGAGGCTAGGTTTGTGGTGGA
GAAAGAAAGCATAAGCGTGCTGAATCCAGAGGAGATGAGGTCGAAGCACGACGGCTCGAT
AGCCAATTTCGGTTTACCCGATTACGGTGGGTTTTTAATCGGGTCAGTGGTTTATCCGGA
TAGTAAAACCGATGGATGCTCTGCTTTTGGTAAAACCTTCAAGCCCAAGTTTCCTCGTCC
CACTATTCTGCTTCTTGATCGTGGAGGTAAAAAAGTTAAAAACATCTTTAAAAGTTAAAA
CCTCTGTTTTGTCCATTTACTGTTGTTTTGTTTGAGTTCAAAGATTTAAACTTTAGTCTG
CTATGTTATTAGCCAATAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTA
CATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATT
TTTTTTCAATTCAAAAATGTAGATGTCCGCACGGTTATT
   
Sequence Name 51-K107370-0022-790-G08C-8409
GenBank Accession LN507522 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>51-K107370-0022-790-G08C-8409
GAGCCTTTACATTGAATATATCCTATTGGCTAATAACATAGCAGACTAAAGTTTAAATCT
TTGAACTCAAACAAAACAACAGTAAATGGACAAAACAGAGGTTTTAACTTTTAAAGATGT
TTTTAACTTTTTTACCTCCACGATCAAGAAGCAGAATAGTGGGACGAGGAAACTTGGGCT
TGAAGGTTTTACCAAAAGCAGAGCATCCATCGGTTTTACTATCCGGATAAACCACTGACC
CGATTAAAAACCCACCGTAATCGGGTAAACCGAAATTGGCTATCGAGCCGTCGTGCTTCG
ACCTCATCTCCTCTGGATTCAGCACGCTTATGCTTTCTTTCTCCACCACAAACCTAGCCT
CGACGACCATAGCGATGATGGTCAACGCCGCGAGGAGAAAGGTCAACGAAGCTCTCCCGT
TGACTAAACCCATCTCTGAAGAATCCACCAAGAAGAGTT

Last Updated on 06.12.2018 16:11