Sequences from confirmation amplicon


Confirmation sequences for line 791B02 and hit At3g61720 are shown.
Line ID 791B02
Locus At3g61720 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:22843139
Primers used for PCR 46vh - 8474, show primer details
   
Sequence Name 66-K117914-0022-791-B02C-46vh
GenBank Accession LN511698 [GenBank]
Sequencing primer gene specific primer, 46vh: 5'-TTCGGTCATTGCATCGGT-3'
Sequence (A. th genome BLAST matches underlined) 
>66-K117914-0022-791-B02C-46vh
TTTTGTGTTTCTTTTTTGGGAGTACTACATATATTGGTTGGTGACTTCGTGGCTAGTTGC
GTATTGCATTGTGCTTTGCATTGTGGTTATACTTTTACGGGAAATCCTCAAGTCCCCGAG
GCAAACATATAACTGGTTGTTTTATCGGAACGTCACTCCGCCTCCTTTGATTCTCGTCGA
TTTGAAGCTACGGAAACTTGACTCGATCAACCTAGACGAGTTAGCCGAAGAGTTTGACTC
GTTTCCGTCGTCGGAGAACGATTTGAACATTCTTAGGATGAGGTACGATAGGTTGAGGAA
GATTATGGAGAACGTCATGTTATTAATGGGGGATGCTGCTACACAAGGCGAACGGTTACT
AGCGGCTTTTACGTTGCTTGAGCGGCCATTCGTTTTGATTATCTTATTGGCCCTCTGTTA
TTGCTCCATGCTCGTCGTTTGCCTTGGTTGGGATCTACATGAATATACAATTGGTAATTG
GCCTTTTAATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAAT
GAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAA
AAATGTAGA
   
Sequence Name 26-K117913-0022-791-B02C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>26-K117913-0022-791-B02C-8409
CTTGTGTACCACCATCCCCCATTAATTAATTGTACGTTCTCCTTAATCTTCCTCAACCTA
TCTTACCTCATCCTAAGAATGTTCACATCGATTTCCA

Last Updated on 06.12.2018 16:11