Sequences from confirmation amplicon


Confirmation sequences for line 791C01 and hit At4g25910 are shown.
Line ID 791C01
Locus At4g25910 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:13164423
Primers used for PCR DA09 - 8474, show primer details
   
Sequence Name 32-K107428-0022-791-C01C-DA09
GenBank Accession LN500611 [GenBank]
Sequencing primer gene specific primer, DA09: 5'-ACCATTCAGATCAATTTCAAAACC-3'
Sequence (A. th genome BLAST matches underlined) 
>32-K107428-0022-791-C01C-DA09
ACAAATACTAACCTTTAAAACAAACCTTCTCAATGTTCTCATCGTTCAGCTCTAAACCTC
CTGTCTCGGATTCAAGAAACTGCTCAACGGACATGATCTCTGGAATCTTGTCTCGAAGAC
GACTCTCGATTCCCATCTTCAACGTCATTGATGAGCTAGGACACGAACCACAAGCTCCTT
GTAGCTTTAAAACCACCACAAGTCCGTCGATTTCGTGCAACGCCACGTTTCCTCCGTCGG
CCATTAGAGATGGTCGTACTTCGTCTAACACTCTCTCCACATTCTCTTCCGTTAACGGCA
TGACGCAGCTCACGTGACCAAATACTAAACCAAACATAACAAGAATCTAAATCACAACTA
AAGTTCTAAAGAGATTGGTCTGGTTTGCAAATCAAGTACCTGTTCGTTTTCTCTACATGG
CTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAA
AGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCACGNTTATTA
   
Sequence Name 16-K107427-0022-791-C01C-8409
GenBank Accession LN493458 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>16-K107427-0022-791-C01C-8409
AGAGAAACGAACAGGTACTTGATTTGCAACCAGACCAATCTCTTTAGAACTTTAGTTGTG
ATTTAGATTCTTGTTATGTTTGGTTTAGTATTTGGTCACGTGAGCTGCGTCATGCCGTTA
ACGGAAGAGAATGTGGAGAGAGTGTTAGACGAAGTACGACCATCTCTAATGGCCGACGGA
GGAAACGTGGCGTTGCACGAAATCGACGGACTTGTGGTGGTTTTAAAGCTACAAGGAGCT
TGTGGTTCGTGTCCTAGCTCATCAATGACGTTGAAGATGGGAATCGAGAGTCGTCTTCGA
GACAAGATTCCAGAGATCATGTCCGTTGAGCAGTTTCTTGAATCCGAGACAGGAGGTTTA
GAGCTGAACGATGAGAACATTGAGAAGGTTTGGTTTTTTAAAGGTTTAGTTATTTTTGGT
TTATTAGCCCCTTGGTTTTGAAATTGATCTGAATGG

Last Updated on 06.12.2018 16:11