Sequences from confirmation amplicon


Confirmation sequences for line 791C05 and hit At5g22070 are shown.
Line ID 791C05
Locus At5g22070 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:7308468
Primers used for PCR 34ve - 8474, show primer details
   
Sequence Name 18-K117874-0022-791-C05C-34ve
GenBank Accession LN494495 [GenBank]
Sequencing primer gene specific primer, 34ve: 5'-AACACTCATCAGAACGATAACACG-3'
Sequence (A. th genome BLAST matches underlined) 
>18-K117874-0022-791-C05C-34ve
GATCTTGATTGTTACAAAGCATGTCTTCTAGTCATAACAAAGAACTGCGAACCAACTCTG
AATTTCTCAAACGGAACCTCAGGCATCATAGCATACCTTCCTCTAGCAGTGTACCGTTTC
CAAAGCCTAGGCTCATCAGATATCAGTTCCATAAAACTCCGATACAAGATTCTAATACCT
CTCGGATTCGGATTCGGATCCGGATCAGATTTATCAAAAATTGAAGATTCAAAGAGAGAG
CTATAAACATAGTTGAACGAGTGGAGAGGAATACAATACTGAGAGAGCACAGCGAAGTAA
GTGTTCGCAGGGTCATCAAGGAAAGCGGTAGCGAGGAGGCGCCGCGTGGCGGAGATAAGA
GTCGGAGAAGCTCTTGCGGTGCGTTTCGCGTTTGCAATGAAGGCATTCTCAAACACAGAG
CCATTCCCAGGTCTTGTGACATTGACAAAAGGATCTGCGTGGACATAGACGTTGTAGAGA
GATTTCGAATGACCAGAGAAGAATCGATCCCAGATTGGCGCGAAATGGAGATCGGAGTTG
GTGAGGAATAGAAAATTACAAAGAAGAAAGAGTAAACAAATTCAATTGTAAATGGCTTCA
TGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAA
GAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGT
   
Sequence Name 06-K117873-0022-791-C05C-8409
GenBank Accession LN488996 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>06-K117873-0022-791-C05C-8409
TTGAATTTGTTTACTCTTTCTTCTTTGTAATTTTCTATTCCTCACCAACTCCGATCTCCA
TTTCGCGCCAATCTGGGATCGATTCTTCTCTGGTCATTCGAAATCTCTCTACAACGTCTA
TGTCCACGCAGATCCTTTTGTCAATGTCACAAGACCTGGGAATGGCTCTGTGTTTGAGAA
TGCCTTCATTGCAAACGCGAAACGCACCGCAAGAGCTTCTCCGACTCTTATCTCCGCCAC
GCGGCGCCTCCTCGCTACCGCTTTCCTTGATGACCCTGCGAACACTTACTTCGCTGTGCT
CTCTCAGTATTGTATTCCTCTCCACTCGTTCAACTATGTTTATAGCTCTCTCTTTGAATC
TTCAATTTTTGATAAATCTGATCCGGATCCGAATCCGAATCCGAGAGGTATTAGAATCTT
GTATCGGAGTTTTATGGAACTGATATCTGATGAGCCTAGGCTTTGGAAACGGTACACTGC
TAGAGGAAGGTATGCTATGATGCCTGAGGTTCCGTTTGAGAAATTCAGAGTTGGTTCGCA
GTTCTTTGTTATGACTAGAAGACATGCTTTGTTAACAATCAAAGATCGGATCTTGTGGCG
TAAATTCAAGCTCCCGTGTTAT
Primers used for PCR 76vs - 8474, show primer details
   
Sequence Name 77-K117997-0022-791-C05C-76vs
GenBank Accession LN513604 [GenBank]
Sequencing primer gene specific primer, 76vs: 5'-CAGGTATTATAACGCTTGTCGGTT-3'
Sequence (A. th genome BLAST matches underlined) 
>77-K117997-0022-791-C05C-76vs
CGCGTCATCACAGATTGATAAAAACAATACTCCGTCATCTTAATCTCTCATCATCACTGA
CTGTGATTGAATTTTTTTTTTTTTTACAACCGGAAACCGGAAAAATTTTTCAAAATCCCC
ATAAAATCCCCCCAAAAATTTCCAACCTTGGGGGATTTCCCCCCAAATTTCGGGAAAAAA
AAAACCTCCGTTTTCCGGGAGTTCCTTTCAATATTTCTCCACCGATTTCGTCCAGTCTTG
GAAAGGGGTCCGCCAACGTATTATCGCCGGAAAAAAGCTAGAAAAATCTTTTTCCCAAAC
CCCCGGAAAGGGGAAACCGAAACAACGCTTTATCTTCAAAATCGGCAAACCCGGGGGAAA
TCCAACCCCATCCGGGGAAATTTTTTCCCGGTTCCGGGGGAAGCCAAAAATTTCGGGGGT
TCCCAATTTTGGTTTCCCTTTCCCAAAGGTCCCCAAACCAGTGGGTCCCCTTCTTCCCCC
TCCCCCAAGGTCCCCCATTGGTTTCCTCCTTGGAAGCCCCCCAGGGGTTCCCTCCCCCCC
CGGGGGAATCTTAAACCCCAACCCCCAAAAAAATGGAGAACCGGAGTTTTTTCTCCCCCG
CGGGGGGATAAACCTCTTTTTCTAATCTCTAATGGGGAAACCCAACCCCAAAGCTCAAAA
AAAAATAACAACCCGCCGGGCCGGGGAAATCTCCGGGGATCACCAAGGAGTAGGAGGGCC
AATAGGGAAAAAAAAAAAGAGTATTTCCCATTTTTTTTCCATTCCAAAAAGGAAAATTCC
   
Sequence Name 37-K117996-0022-791-C05C-8409
GenBank Accession LN502773 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>37-K117996-0022-791-C05C-8409
GATCTAATTTGTTCTTGAGCTTAGGGTTAGGGTTTCCAGATGAGAGATGAGAAAGAGAGG
TTGATCCAGCAGCGGCGATGAATAAACTCCGGTCATCAATCTCATCGGAGATTGGGATTA
AAGATTCACCAGGAGGAGAAGGAAACACATGTGGCGCTACAAAGAAGAAAACAAATGGGA
GACATAGGAGAAGAGAGAAGAAGAGAACAAACTGATTCGAGAACATTTGGAAAAGGAAAA
CAAAAATCGGAAACCCTAGAAATTTTTGACTTCCCCAGAAACTGGAAAAAAATCTACCGG
AATGTGATTGAATCTCCCATGGATTAGCAGATTCTGAAGATAAAGCGTTGTCTCGATTTC
ACATTACTGGAGCTATGAGAAATAGATTCTCATAGCTTTTTTCCGACGATAATACGTCGA
CGAACCTCTCTCAAAGACTGAACGAAATCGATGAAGAAATATAGAAAGAAACTCCTGAAA
AACGAAGGTTATCTATCTCCAGAAATTTGGGGGAAAATCAGCAAAGATTGAAAATTTATG
GGGGAATTTAATGAGAATTTAGAAATATCTTTCAGATCTCAGATTGTAAAAAAAAAAAAA
AATTCAATCCCGGTCGGGGATGATGAAAAATTAAAATGACGGATTATTGTTTTTAACCAA
TCGGGGATTGACCCCCCCTTTTGGCCGAGGGGGTTTTTCCGTCATTTTACAAAACCAACA
AG

Last Updated on 06.12.2018 16:11