Sequences from confirmation amplicon


Confirmation sequences for line 791F01 and hit At5g03435 are shown.
Line ID 791F01
Locus At5g03435 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:854560
Primers used for PCR 36vh - 8474, show primer details
   
Sequence Name 12-K117806-0022-791-F01C-36vh
GenBank Accession LN491774 [GenBank]
Sequencing primer gene specific primer, 36vh: 5'-TGCTCTCATCTCTTCGATCTGATA-3'
Sequence (A. th genome BLAST matches underlined) 
>12-K117806-0022-791-F01C-36vh
TTTCGTACAACCAACGGAAAGGAGCTGAATACCGTGCATACCCTTGCGCAACATAAACAA
AGCGAACCGCGAGCTGAAGCTCTCCCATTTTCTTCAGTCCGCTGCTTCCCAACTTCAGAA
TTGGATACGAGCAAGCATAAATCCAGTCACTTTCTACACGGTTCAATGAAATCCTCACTT
TCCCGATCGGAACATCGTTCGCCTGATCCTCTTTAAAGATTTGTCGATTGTCGTAGATTC
CAAGGGTTAATACCGTACACTTCTCGTAATCATCCCACGAATACTGCTCGTTCCACTTGG
GTGTGACACTATTAACCACGGTTCGAGTCCTGGCCCATTTATTCCCGTATGTAGTATATG
GGCATCATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGA
GTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAA
ATGTAGATATCCGCA
   
Sequence Name 12-K117784-0022-791-F01C-8409
GenBank Accession LN491773 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>12-K117784-0022-791-F01C-8409
TTTCATTGAATTGATGCCCATATACTACATACGGGAATAAATGGGCCAGGACTCGAACCG
TGGTTAATAGTGTCACACCCAAGTGGAACGAGCAGTATTCGTGGGATGATTACGAGAAGT
GTACGGTATTAACCCTTGGAATCTACGACAATCGACAAATCTTTAAAGAGGATCAGGCGA
ACGATGTTCCGATCGGGAAAGTGAGGATTTCATTGAACCGTGTAGAAAGTGACTGGATTT
ATGCTTGCTCGTATCCAATTCTGAAGTTGGGAAGCAGCGGACTGAAGAAAATGGGAGAGC
TTCAGCTCGCGGTTCGCTTTGTTTATGTTGCGCAAGGGTATGCACGGTATTCAGCTCCTT
TCCGTTGGTTGTTACCGAAAGCTCATTACAAATCTCCATTGTCGGTGTATCAGATCGAAG
AGATGAGAGC

Last Updated on 06.12.2018 16:11