Sequences from confirmation amplicon


Confirmation sequences for line 793E04 and hit At4g20720 are shown.
Line ID 793E04
Locus At4g20720 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:11105627
Primers used for PCR 10vb - 8474, show primer details
   
Sequence Name 40-K117566-0022-793-E04C-10vb
GenBank Accession LN503853 [GenBank]
Sequencing primer gene specific primer, 10vb: 5'-AGCATCTTTCTCCTCAAAGAA-3'
Sequence (A. th genome BLAST matches underlined) 
>40-K117566-0022-793-E04C-10vb
CTTTCCTGCGCATCTCGTCCAGCAAACAAACTAAGATTCTCATGTTCCCCAGAAGAAACA
ACACTCTTAGGTGCATCTTTTTTATCAAACAAACCAACATTATCATGCTGTTGCGATCCA
ACAACTCCCTGACTCTTTACACTATCGAATAAGCTAAGACTACGTGGATCCTTAAAATCA
TCATCCTCCACAGGTTTACTCTCTGCCTGCTCCACTTTGGAAAGATCTCCTCTCCGCTCA
ACGAAATAATCATCAAGATTAATCCCGCCTAGATTCAGAGGGTTTTTCTTCTGTACTGAT
TGTCCATCATCAGACTTCTCCTCTAATGCAGACCATTGTATTTCCAAATCAAGAAACTTA
GACAAAGCAATTCCTTTTGAAGGCGGTGCCTTAGTAGCTCCTCTGCTTGACCCATCCGTT
TCCTTTAGTGGCTCTACCATCTCCTACAGATGAAAAGTTCGTTTTTTTGAAGACACAACA
CACCACATTCTACATCTGACTAATACACAATCAGCTAGGAAAACAAGTAATTACAAATTA
GTGAGAAAGGTATCGACTTTTACCGATCCATCGAGATTAAGAGAGGTGAGAAACCATTTG
TAAGCAGAAGTGGAAGTAAATTTGATGGGATCAGGAGGATTATCACTCGTGCGTTGCTGA
TTTCCATCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCA
GCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAA
TTCAAAAATGTAGATGTCCGC
   
Sequence Name 08-K117565-0022-793-E04C-8409
GenBank Accession LN489911 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>08-K117565-0022-793-E04C-8409
ATTTCATTGAATAATCCTGATGGAAATCAGCAACGCACGAGTGATAATCCTCCTGATCCC
ATCAAATTTACTTCCACTTCTGCTTACAAATGGTTTCTCACCTCTCTTAATCTCGATGGA
TCGGTAAAAGTCGATACCTTTCTCACTAATTTGTAATTACTTGTTTTCCTAGCTGATTGT
GTATTAGTCAGATGTAGAATGTGGTGTGTTGTGTCTTCAAAAAAACGAACTTTTCATCTG
TAGGAGATGGTAGAGCCACTAAAGGAAACGGATGGGTCAAGCAGAGGAGCTACTAAGGCA
CCGCCTTCAAAAGGAATTGCTTTGTCTAAGTTTCTTGATTTGGAAATACAATGGTCTGCA
TTAGAGGAGAAGTCTGATGATGGACAATCAGTACAGAAGAAAAACCCTCTGAATCTAGGC
GGGATTAATCTTGATGATTATTTCGTTGAGCGGAGAGGAGATCTTTCCAAAGTGGAGCAG
GCAGAGAGTAAACCTGTGGAGGATGATGATTTTAAGGATCCACGTAGTCTTAGCTTATTC
GATAGTGTAAAGAGTCAGGGAGTTGTTGGATCGCAACAGCATGATAATGTTGGTTTGTTT
GATAAAAAAGATGCACCTAAGAGTGTTGTTTCTTCTGGGGAACATGAGAATCTTAGTTTG
TTTGCTGGACGAGATGCGCAGGAAAGTGTTTCTTTCGCAGCGCAGGGAAACTTTGGGTTC
TTTGAGGAAAAAGATGCT
Primers used for PCR 53vu - 8474, show primer details
   
Sequence Name 50-K117995-0022-793-E04C-53vu
GenBank Accession LN507308 [GenBank]
Sequencing primer gene specific primer, 53vu: 5'-TGTTCTTTCCCTTTGATTATTTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>50-K117995-0022-793-E04C-53vu
ATTCATTGTTTGATTACGAATCAACAGTAGAGACGGGCTCTATAAGCATGTGAGATGTGC
TATTTTCCCAAAATGATAGATGTTCAAGGTGTGTTTTTTTATTTGTACAATGAGATTAAT
AAATCATTTATTTTTAGTCAAGAAGCATCGAAGCAAGAGAACGAACCATTTGAAAGACTC
ATAAACCAAATTGAGTCAAAGGAATTTCAAAGAAGTAGTTTGGTATGGACGGATCTGGTA
AACCGGTTTCGGTACGATTGTCTGGTTATTTTTGGTTTAAAATTGATTGGGTATGTTGCA
GAAGCTTTGCCTCAGATGGCAGGTCTCTCCAAATTGATCATCCTTCGATATTATTATTGA
TACAGAGGAGGAAGACTCAGAATCTTCATCCTCAAACCCTAGATTCCTTAACGAAATGGC
GATGGAGATCTCGGTAGATCTGATAAACCAACTCAAGGTCTCGCTTCGAAAGGAGGCCAA
GCTCACCTCCGTCGACGATTGCTCGGACTCATCCTTCCCCAGCCTTCCCACATCCGAGGA
GGCTATCGCAGAACTTGATGCTTCAGCTCCCTACCTTCGTTGCCGAAACTGCAAAGGAAA
ACTTTTACGAGGAATCGAGTCTCTGATCTGTGTCAGGATATATTCAATTGTAAATGGCTT
CATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGA
AAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCC
   
Sequence Name 10-K117994-0022-793-E04C-8409
GenBank Accession LN490856 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>10-K117994-0022-793-E04C-8409
TTTCATTGAATTATCCTGACACGATCAGAGACTCGATTCCTCGTAAAAGTTTTCCTTTGC
AGTTTCGGCAACGAAGGTAGGGAGCTGAAGCATCAAGTTCTGCGATAGCCTCCTCGGATG
TGGGAAGGCTGGGGAAGGATGAGTCCGAGCAATCGTCGACGGAGGTGAGCTTGGCCTCCT
TTCGAAGCGAGACCTTGAGTTGGTTTATCAGATCTACCGAGATCTCCATCGCCATTTCGT
TAAGGAATCTAGGGTTTGAGGATGAAGATTCTGAGTCTTCCTCCTCTGTATCAATAATAA
TATCGAAGGATGATCAATTTGGAGAGACCTGCCATCTGAGGCAAAGCTTCTGCAACATAC
CCAATCAATTTTAAACCAAAAATAACCAGACAATCGTACCGAAACCGGTTTACCAGATCC
GTCCATACCAAACTACTTCTTTGAAATTCCTTTGACTCAATTTGGTTTATGAGTCTTTCA
AATGGTTCGTTCTCTTGCTTCGATGCTTCTTGACTAAAAATAAATGATTTATTAATCTCA
TTGTACAAATAAAAAAACACACCTTGAACATCTATCATTTTGGGAAAATAGCACATCTCA
CATGCTTATAGAGCCCGTCTCTACTGTTGATTCTGTAATCAAACAATGAATTTGTACTAA
GGGATTTAGACAAATTCACTCCTCTCTTAGCTCGCAAATAATCAAAGGGAAAGAAC

Last Updated on 06.12.2018 16:11