Sequences from confirmation amplicon


Confirmation sequences for line 794A09 and hit At5g02630 are shown.
Line ID 794A09
Locus At5g02630 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:592661
Primers used for PCR AT13 - 8474, show primer details
   
Sequence Name 59-K117029-0022-794-A09C-AT13
GenBank Accession LN510089 [GenBank]
Sequencing primer gene specific primer, AT13: 5'-ACTTTAGCTTCTGCGTTCTTGATT-3'
Sequence (A. th genome BLAST matches underlined) 
>59-K117029-0022-794-A09C-AT13
CTCCTCGCTCCAGTTTCAGTAAATCTTATCCGATAACGTCTCCAAACGATTACTCTCTGT
TCTTCGCTAATTGCGTACCAGAAACTAGGGTTTCGATGAAGGTCCACACGGAGATTTATC
ACGATCTATATCCAAACGGGTCAAGAGATTACTTACTAGCCGGGTCAGCCCAGTTACCGG
GTTTGTATCTCGTCTTTTTTCTCTGTTACTTGAGTTTCCTCTGTTTCTGGCTCTGTTTCT
GCTGGAATCATAAGCAAATTGTTAAAAGGATTCACCTTTTAATGACGGCTCTGCTTCTAG
TGAAATCTCTGACTCTGATTTGTGCTGCTGTGTATAAACACTACGTGAAAGTCACAGGCA
CAGCTCATGGCTGGAACATTGTTTTCTACATTTTCCAATTCATTAGTGTTGTTTTGCTCT
TCATGGTGATTGTTCTAATCGGAAACGGTTGGTCTTTCTTGAAACCGAAGCTACATGTTA
AGGAGAAGAAATTGTTGGTGATTGTTGTTCCGCTTCAGGTTTTAGCTAACATTGCTTCCA
TTGTGATTGGAGAGACTGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGG
AGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTA
   
Sequence Name 27-K117028-0022-794-A09C-8409
GenBank Accession LN498563 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>27-K117028-0022-794-A09C-8409
AAGGTTAGCTAAAACCTGAAGCGGAACAACAATCACCAACAATTTTCTTTCTCCCTTAAC
ATGTAGCTTCGGTTTTCAAGAAAGACCAACCNGTTTCCGATTAGAACAATCACCATGAAG
AGCAAAACAACACTAATGAATTGGAAAATGTAGAAAACAATGTTCCAGCCATGAGCTGTG
CCTGTGACTTTCACGTAGTGTTTATACACAGCAGCACAAATCAGAGTCAGAGATTTCACT
AGAAGCAGAGCCGTCATTAAAAGGTGAATCCTTTTAACAATTTGCTTATGATTCCAGCAG
AAACAGAGCCAGAAACAGAGGAAACTCAAGTAACAGAGAAAAAAGACGAGATACAAACCC
GGTAACTGGGCTGACCCGGCTAGTAAGTAATCTCTTGACCCGTTTGGATATAGATCGTGA
TAAATCTCCGTGTGGACCTTCATCGAAACCCTAGTTTCTGGTACGCAATTAGCGAAGAAC
AGAGAGTAATCGTTTGGAGACGTTATCGGATAAGATTTACTGAACTGGGAGCGAGGAGGA
GGAGAGAGATCGACGAAGGTGAAGAAATGAAGTATGTAGTGTGAATCAAGACC

Last Updated on 06.12.2018 16:11