Sequences from confirmation amplicon


Confirmation sequences for line 795B10 and hit At4g01180 are shown.
Line ID 795B10
Locus At4g01180 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:501698
Primers used for PCR 47uj - 8474, show primer details
   
Sequence Name 30-K117387-0022-795-B10C-47uj
GenBank Accession LN499892 [GenBank]
Sequencing primer gene specific primer, 47uj: 5'-GGAAACAATTTCTCAGCTC-3'
Sequence (A. th genome BLAST matches underlined) 
>30-K117387-0022-795-B10C-47uj
ATTATCATCACAACCAAGATAAGAAAAACTAATTGAAACTGGAAACTTAACAAAAAGGGA
CAACATTTTCCTAAGGCTCTTCATAGTTGTCTCCTCAAGGCCTCTTTCGTTTGTTCTTCA
TAGCAGCAAGTTTGGACCTTATCTTCAACATCACATCTGTTATTTCCTCGATAGTCGCCT
TTCGGTTCTCCTCATAGTTCCAAAGCTCTTCAGTCTCATAGCTGCCACTAGCGTTATGTT
CCTCCATCTCTAGCTTTGCTCTTACCACTTCACTATAAACTTCTTCACCGTACTGGTTCT
TCAGCATACGAAGCTTCTCATCATTTTCATCCACCACTCTCTGCATTTCACATTTGGAAT
GTTGAGATATATGTAATTTTTGCTACAAACCCATCTTTTGAGATTTATGAAAGTGTAGAC
TCCTAAGTTAAAGTCCTAACCTTTGGTGTTCCATCAGACTCATCGACCCTAAATGGAGTC
CATTGCACATCTCCTATCTCTGCTGCAATTGTAAATGGCTTCATGTCCGGGAAATCTACA
TGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTT
TTTTCAATTCAAAAATGT
   
Sequence Name 03-K117386-0022-795-B10C-8409
GenBank Accession LN487659 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>03-K117386-0022-795-B10C-8409
TTACATTGCAGCAGAGATAGGAGATGTGCAATGGACTCCATTTAGGGTCGATGAGTCTGA
TGGAACACCAAAGGTTAGGACTTTAACTTAGGAGTCTACACTTTCATAAATCTCAAAAGA
TGGGTTTGTAGCAAAAATTACATATATCTCAACATTCCAAATGTGAAATGCAGAGAGTGG
TGGATGAAAATGATGAGAAGCTTCGTATGCTGAAGAACCAGTACGGTGAAGAAGTTTATA
GTGAAGTGGTAAGAGCAAAGCTAGAGATGGAGGAACATAACGCTAGTGGCAGCTATGAGA
CTGAAGAGCTTTGGAACTATGAGGAGAACCGAAAGGCGACTATCGAGGAAATAACAGATG
TGATGTTGAAGATAAGGTCCAAACTTGCTGCTATGAAGAACAAACGAAAGAGGCCTTGAG
GAGACAACTATGAAGAGCCTTAGGAAAATGTTGTCCCTTTTTGTTAAGTTTCCAGTTTCA
ATTAGTTTTTCTTATCTTGTGTTGTTGATTGATAATATATCGTAGAGTCTTAGATCACAA
GAAATGGAGCTGAGAATTTTGTTT

Last Updated on 06.12.2018 16:11