Sequences from confirmation amplicon


Confirmation sequences for line 796D03 and hit At2g02730 are shown.
Line ID 796D03
Locus At2g02730 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:765596
Primers used for PCR NF31 - 8474, show primer details
   
Sequence Name 41-K102431-0022-796-D03C-8409
GenBank Accession LN504030 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>41-K102431-0022-796-D03C-8409
CCATTACTCGTTTATATTGAAGCCATTGGTAATCTAGATGTCTCACCTCTGAACTTCCTA
CATCTTGTCCATAGACTTTTATGATAACTTGCTTCATGTGGTCTTGCAGGAGAAGCTTCG
GCTTTCAGGGAAGTCAAACACATGTATAGTACTTACAACCGAAGAAACTTCAAGCTCAGA
GGTATTTTGGATCAATATATATGATATCAAGTACACTCGCAGATAGAATCAGATGCAGCA
TTGCAGTATGATTGTATTAAACCTTCAAAACTCATCCGATCTGTCTCTTGAAATGTTTCA
GGGTTTAAAAGAAAGCGATAAGATACAGCTACTAGATGACTGCTAATTCAAATGGTGCCA
ATATATCATCAAGGTTTGAGGTCATATTACTTCTTTCTTT
   
Sequence Name 41-K102432-0022-796-D03C-NF31
GenBank Accession LN504031 [GenBank]
Sequencing primer gene specific primer, NF31: 5'-GTCCCTGTTCATGAAAGAAAGAAG-3'
Sequence (A. th genome BLAST matches underlined) 
>41-K102432-0022-796-D03C-NF31
TATTGGCACCATTTGAATTAGCAGTCATCTAGTAGCTGTATCTTATCGCTTTCTTTTAAA
CCCTGAAACATTTCAAGAGACAGATCGGATGAGTTTTGAAGGTTTAATACAATCATACTG
CAATGCTGCATCTGATTCTATCTGCGAGTGTACTTGATATCATATATATTGATCCAAAAT
ACCTCTGAGCTTGAAGTTTCTTCGGTTGTAAGTACTATACATGTGTTTGACTTCCCTGAA
AGCCGAAGCTTCTCCTGCAAGACCACATGAAGCAAGTTATCATAAAAGTCTATGGACAAG
ATGTAGGAAGTTCAGAGGTGAGACATCTAGATTACCAATGGCTTCAATATAAAACGAGTA
AATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGA
GAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGT
TATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAG
GCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTACATTC

Last Updated on 06.12.2018 16:11