Sequences from confirmation amplicon


Confirmation sequences for line 797F08 and hit At1g25440 are shown.
Line ID 797F08
Locus At1g25440 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:8934800
Primers used for PCR GA55 - 8474, show primer details
   
Sequence Name 11-K101382-0022-797-F08C-8409
GenBank Accession LN491046 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>11-K101382-0022-797-F08C-8409
CATTTACATTGAATATATCCTGATCATAGGAAACCACTTCTGATCATAGGAAACTCGATC
TTGGGCTCAACGACATCGTTCAAGAACTGCTCAGACACCAACGGATCTAGAACCGGCACT
TGACAGATCAGCTGCTCTTCAAGCTCATAGTTGTCTGTCTGATCCTCAATACTAATATCA
GGAACCAAGTCATGAAATATCGACGAATTGTTTTTCTTACCAGAGCCACGTGGCGTTCGA
GCTTTACGAGTAAACCCGTGATGCCACGTGGCGACCTCATGTGGAGGAGAAGCTGATGAG
TGGTTGCTATGCTTTACGACCGCCGGGCTAGCCGTCTTCAAACGGACTCTCTCGTGGCGG
CGAGCAAGAGGGTTTGCTGAATGGACCAAACTGTCGCAAGACTGGCAAAGAAAAGCATCG
TCGGCCGCGCAGTACCACCGTGCACGTCTCTTCACGCAGCTGTCGCAAGCCCTCGCCGTC
TTCGCTCCAACAGCATTCGCCAAACTTTTCATCATTGTATACAAAGTCTACACAACCAAC
ATCAACGACNAGAGCACGAGAGCTTTGGTTTTGAGATCAATCTCAATTATNGAATCTTTT
GCTCTCTGCAAAAGTTAGATTTAGTGTAGTCCTAGAGAGTTTGAGATTAAGAGGATGACC
CGCATGAAGCGCATTCCCTTTTGTGTCAGT
   
Sequence Name 11-K101388-0022-797-F08C-GA55
GenBank Accession LN491047 [GenBank]
Sequencing primer gene specific primer, GA55: 5'-TTCAAAAATCCACTGACACAAAAG-3'
Sequence (A. th genome BLAST matches underlined) 
>11-K101388-0022-797-F08C-GA55
TCTTATCTCAACTCTCTAGGACTACACTAAATCTAACTTTTTGCAGAGAGCAAAAGATTC
AATAATTGAGATTGATCTCAAAACCAAAGCTCTCGTGCTCTTGTCGTTGATGTTGGTTGT
GTAGACTTTGTATACAATGATGAAAAGTTTGGCGAATGCTGTTGGAGCGAAGACGGCGAG
GGCTTGCGACAGCTGCGTGAAGAGACGTGCACGGTGGTACTGCGCGGCCGACGATGCTTT
TCTTTGCCAGTCTTGCGACAGTTTGGTCCATTCAGCAAACCCTCTTGCTCGCCGCCACGA
GAGAGTCCGTTTGAAGACGGCTAGCCCGGCGGTCGTAAAGCATAGCAACCACTCATCAGC
TTCTCCTCCACATGAGGTCGCCACGTGGCATCACGGGTTTACTCGTAAAGCTCGAACGCC
ACGTGGCTCTGGTAAGAAAAACAATTCGTCGATATTTCATGACTTGGTTCCTGATATTAG
TATTGAGGATCAGACAGACAACTATGAGCTTGAAGAGCAGCTGATCTGTCAAGTGCCGGT
TCTAGATCCGTTGGTGTCTGAGCAGTTCTTGAACGATGTCGTTGAGCCCAAGATCGAGTT
TCCTATGATCAGAAGTGGTTTCCTATGATCAGGATATATTCAATTGTAAATGGCTTCATG
TCCGGGGAATCTACATGGATCAGNCATGAGTATGATGGTCAATATGGAGAAAAAGAAAGA
GTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCA

Last Updated on 06.12.2018 16:11