Sequences from confirmation amplicon


Confirmation sequences for line 798E08 and hit At5g04850 are shown.
Line ID 798E08
Locus At5g04850 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:1408720
Primers used for PCR 24dr - 8474, show primer details
   
Sequence Name 55-K109780-0022-798-E08C-24dr
GenBank Accession LN508841 [GenBank]
Sequencing primer gene specific primer, 24dr: 5'-AAAATAGCACATTGAACCAAAACC-3'
Sequence (A. th genome BLAST matches underlined) 
>55-K109780-0022-798-E08C-24dr
GCAGATGACAGCGCTAAAATCTGCTAACAAGGAGCTGAAAGGAATGATGAAAACCGTCAA
CATTCAAGATATAGATGTATGTCTTGGTTCTTATTCAAGCTTCTCTTGTGTGTTTGCTTC
CTAGATACTGTTTTGGTTAAGAATTATTTGTTGTATACCACAGAATTTGCAAGATGATAT
GATGGACTTAATGGATGAGAGTTCTGAAATTCAAGAGACACTTGGTAGGAGCTACAATGT
TCCTGATGACATTGACGAAGATGACCTCCTGGGCGGTAAGTTCTTTCTCTGCTCTTTATG
CTGCTTGTCCTTGGGGTTGGTAGAAACACTTTCCCCG
   
Sequence Name 39-K109779-0022-798-E08C-8409
GenBank Accession LN503384 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>39-K109779-0022-798-E08C-8409
GCCATTTACAATTGAATATATCCTGCATCCAATCAAGATGATAAACCGATATCACGTATA
AGGAGCAGAATTTAACAAACACAGGGAAATTGTTTCTACCAACCCCAAGGACAAGCAGCA
TAAAGAGCAGAGAAAGAACTTACCGCCCAGGAGGTCATCTTCGTCAATGTCATCAGGAAC
ATTGTAGCTCCTACCAAGTGTCTCTTGAATTTCAGAACTCTCATCCATTAAGTCCATCAT
ATCATCTTGCAAATTCTGTGGTATACAACAAATAATTCTTAACCAAAACAGTATCTAGGA
AGCAAACACACAAGAGAAGCTTGAATAAGAACCAAGACATACATCTATATCTTGAATCTT
GACGGTTTTCATCATTCCTTTCAGCTCCTTGTTAGCAGATTTTAGCGCTGTCATCTGCAT
ATTCATTCATGTACATGAGGTTTTGGTTCCATGTGCTATTTT

Last Updated on 06.12.2018 16:11