Sequences from confirmation amplicon


Confirmation sequences for line 799A05 and hit At5g51860 are shown.
Line ID 799A05
Locus At5g51860 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:21083624
Primers used for PCR BM90 - 8474, show primer details
   
Sequence Name 38-K104418-0022-799-A05C-BM90
GenBank Accession LN502968 [GenBank]
Sequencing primer gene specific primer, BM90: 5'-GATTATATGAATTCGCTAGCTCCG-3'
Sequence (A. th genome BLAST matches underlined) 
>38-K104418-0022-799-A05C-BM90
ATTCGCTTGATATTCTCCATCTTTATTATACTATTTCATATTTTTTTATGATGTTGATAT
TTGTTGTCATTATAGTACTTGTGTAGTGTAATATCTTTCAGACCAAGTAGTACTAGAGAT
ATCTAGGTAAGATTTCCCAAAATTTGCATCTTTCAATTTTATTTTTATATTATGTTCTAT
AGATTTTTTTTGTTAACAATTATTTTCCTCTTTTTACTTCCGGTTCTTTTTACATTTTAT
AGTTCCACTTGTTGTTCATATTATGAATTAATTAGGGTTTTCAGAAATATAGATTATTGA
ATCAATTGAATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGC
AATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATT
CAAAAATGTAGATGTCCGCACGGATTATT
   
Sequence Name 54-K104419-0022-799-A05C-8409
GenBank Accession LN508470 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>54-K104419-0022-799-A05C-8409
ATTTACAATTGAATAATTCAATTGATTCAATAATCTATATTTCTGAAAACCCTAATTAAT
TCATAATATGAACAACAAGTGGAACTATAAAATGTAAAAAGAACCGGAAGTAAAAAGAGG
AAAATAATTGTTAACAAAAAAAATCTATAGAACATAATATAAAAATAAAATTGAAAGATG
CAAATTTTGGGAAATCTTACCTAGATATCTCTAGTACTACTTGGTCTGAAAGATATTACA
CTACACAAGTACTATAATGACAACAAATATCAACATCATAAAAAAATATTGAAATAGTAT
AATAAAGATTGGAGTAATTAATCAAGCTGAAATTAAAAATCTTACTCGGAGCTAGCGAAT
TCATATAA

Last Updated on 06.12.2018 16:11