Sequences from confirmation amplicon


Confirmation sequences for line 799D01 and hit At3g22790 are shown.
Line ID 799D01
Locus At3g22790 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:8052607
Primers used for PCR 45bk - 8474, show primer details
   
Sequence Name 52-K107839-0022-799-D01C-45bk
GenBank Accession LN507838 [GenBank]
Sequencing primer gene specific primer, 45bk: 5'-TTGAATCACAGTCTGAGAAAATGG-3'
Sequence (A. th genome BLAST matches underlined) 
>52-K107839-0022-799-D01C-45bk
CTAGAAGCCTGAGGACATGCAAAGATTCTGGAGAGACTTTTGTCTGATTCTCGAAGATTG
GCAAGCCTTAGAATCAGCCTAAGAGATCTGAAAAGCAAGCTGGAGATAAACGAGAAGCCA
GGCAAGTTCACTAACCCTGATTTTGCTAGAGTGAGGAAACAGATGAAAGAAATGGAAGAA
GCCATCTTTCAGCTTGCAAACACAAATGAGATTCTTTCGAACGAAATTGAGGAGACCGGA
GATGTCAGAGATATCTATAGAAAAGTGGTTATGGAGAAGTCAAGGATTGGGTCTGAGAAG
ATAGAGCAGATGCAGCAAGAAATGCAGAACATTGAGCGAACAGTGCTGAAGCTTGAAGAG
GGAGCAACTCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAG
TAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTA
   
Sequence Name 84-K107840-0022-799-D01C-8409
GenBank Accession LN514439 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>84-K107840-0022-799-D01C-8409
GCTTCGCACTGTTCGCTCAATGTTCTGCATTTCTTGCTGCATCTGCTCTATCTTCTCAGA
CCCAATCCTTGACTTCTCCATAACCACTTTTCTATAGATATCTCTGACATCTCCGGTCTC
CTCAATTTCGTTCGAAAGAATCTCATTTGTGTTTGCAAGCTGAAAGATGGCTTCTTCCAT
TTCTTTCATCTGTTTCCTCACTCTAGCAAAATCAGGGTTAGTGAACTTGCCTGGCTTCTC
GTTTATCTCCAGCTTGCTTTTCAGATCTCTTAGGCTGATTCTAAGGCTTGCCAATCTTCG
AGAATCAGACAAAAGTCTCTCCAGAATCTTTGCATTGTCCTCAGTGCTTCTAGATAACTC
TAGTTTGTCAACAACTCCAACCATTTTCTCAGACTGTGATTCA

Last Updated on 06.12.2018 16:11