Sequences from confirmation amplicon


Confirmation sequences for line 799H01 and hit At4g04740 are shown.
Line ID 799H01
Locus At4g04740 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:2407185
Primers used for PCR MJ53 - 8474, show primer details
   
Sequence Name 53-K029917-022-799-H01C-8409
GenBank Accession LN508045 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>53-K029917-022-799-H01C-8409
CATTACAATTGATTTTGGGGTTTCTCTGTTCATTGAAGATTGATCATATTCTTTCACGAA
TCGAAAATATGCACTTTGTGCTTTAATCATGAGCTAGATTGGTGACTTATGTTCCAATGC
ATAGAGATGGGACTCTACTCTTTAGTAATAGAATCAGGTTTATGTTGTCTTATTATTGTA
CAGATTTAACTATGTTTATGTTTTCGTTTTAGTTTATTTTGATTTTTATGTTTATCTGTT
TGTGAAACCAAAATTACAGGAAAAATTTACAAGGATGTGGTTGGGAGTCCTTACTACGTT
GCTCCTGAAGTATTAAGGCAGAGTTACGGAAAGNAAAATCG
   
Sequence Name 53-K029917-022-799-H01C-MJ53
GenBank Accession LN508046 [GenBank]
Sequencing primer gene specific primer, MJ53: 5'-GTCGATTTCTTTTCCGTAACTCTG-3'
Sequence (A. th genome BLAST matches underlined) 
>53-K029917-022-799-H01C-MJ53
AACCACATCCTTGTAAATTTTTCCTGTAATTTTGGTTTCACAAACAGATAAACATAAAAA
TCAAAATAAACTAAAACGAAAACATAAACATAGTTAAATCTGTACAATAATAAGACAACA
TAAACCTGATTCTATTACTAAAGAGTAGAGTCCCATCTCTATGCATTGGAACATAAGTCA
CCAATCTAGCTCATGATTAAAGCACAAAGTGCATATTTTCGATTCGTGAAAGAATATGAT
CAATCTTCAATGAACAGAGAAACCCCAAAATCAATTGTAAATGGCTTCATGTCCGGGAAA
TCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACC
AATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACAT
TTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTA
TTCTAATTCGGAAATCTTTATTTCGACGTGTCTACATTC

Last Updated on 06.12.2018 16:11