Sequences from confirmation amplicon


Confirmation sequences for line 799H03 and hit At1g75500 are shown.
Line ID 799H03
Locus At1g75500 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:28338856
Primers used for PCR HV64 - 8474, show primer details
   
Sequence Name 59-K101327-0022-799-H03C-8409
GenBank Accession LN509945 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>59-K101327-0022-799-H03C-8409
GAGCCATTTACATTGAATATATCCTGAGTGAAAAGCTCCCAACCGGAGTGAAAAACCCAA
GCCTGAGAATCTCTTTCACAGAAAGCAGCAATGATCAAGAACTGAATGATTCCGAAGAAA
CAAGTGTAAGACGTAACCGAGAGCCTCGCTGGATAAGACTTAAGAACCGGAGCTTGGAAA
ACAAGCCAGCCTGACCACGAGAGACAGTGACCGATTAGGTAGATGCAACCAAGGGTCCAG
TTTTTAGGCGCGGCGTTTCCTAGCGGCGCTAAGACGGCGGAGTTTGTGGTGAGTAGATGA
GCGTGGAGGTGGCTAGCCGGAGTGTAGATGGTTGGACCTTTATAGAGGGTGATGACGGAA
GCTCCGGCGACACAAAGAGCTGTTCCTAAGATTTTGGAGATACCGTCTCTTCTG
   
Sequence Name 59-K101333-0022-799-H03C-HV64
GenBank Accession LN509946 [GenBank]
Sequencing primer gene specific primer, HV64: 5'-ACAGAAGAGACGGTATCTCCAAAA-3'
Sequence (A. th genome BLAST matches underlined) 
>59-K101333-0022-799-H03C-HV64
TCATCACCCTCTATAAAGGTCCAACCATCTACACTCCGCCTATAAACCTCCACGCTCATC
TACTCACCACAAACTCCGCCTTCTTAGCGCCGCTAGGAAACGCCGCGCCTAAAAACTGGA
CCCTTGGTTGCATCTACCTAATCGGTCACTGTCTCTCGTGGTCAGGCTGGCTTGTTTTCC
AAGCTCCGGTTCTTAAGTCTTATCCAGCGAGGCTCTCGGTTACGTCTTACACTTGTTTCT
TCGGAATCATTCAGTTCTTGATCATTGCTGCTTTCTGTGAAAGAGATTCTCAGGCTTGGG
TTTTTCACTCCGGTTGGGAGCTTTTCACTCAGGATATATTCAATTGTAAATGGCTTCATG
TCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGA
GTAATTACCAATTTTTTTTCAATTTCAAAATG

Last Updated on 06.12.2018 16:11