Sequences from confirmation amplicon


Confirmation sequences for line 800D11 and hit At1g18610 are shown.
Line ID 800D11
Locus At1g18610 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:6405696
Primers used for PCR 26gz - 8474, show primer details
   
Sequence Name 62-K112667-0022-800-D11C-26gz
GenBank Accession LN510795 [GenBank]
Sequencing primer gene specific primer, 26gz: 5'-AGACATGCAATATGGATGAACAAA-3'
Sequence (A. th genome BLAST matches underlined) 
>62-K112667-0022-800-D11C-26gz
TCGAATCCGAATGGGAATAACCCAAATCCGCCATTTTTTGAGAACAGAGACGATGTGTAA
AGAGAGAACTAACCAGCGTCGAAAACATGGACTTGATTGGTTTGGCAATTATCTCTGCCA
TAGCCACCGAAAACATAGAGAAAGCTACCTCCTTTAATGGCGTTACAAGTGTGACCCCAC
CTCTTACCGGGCCCGGAAGAAGACTCACCGAGACCCACTTGTTGCTGTAGTTGTCGGACC
CTTTCCCACCTCATTTTCCGGGCGAGAATCGTTTGGGGTGAGAAAGTGCGGGAAAATGAA
ATGGGTTCTTTTTTGGAGGAGGAGGAGGAATGAGGAAATCTACATGGCTTCATGTCCGGG
AAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATT
ACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATT
   
Sequence Name 30-K112666-0022-800-D11C-8409
GenBank Accession LN499789 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>30-K112666-0022-800-D11C-8409
ATTTCCTCATTCCTCCTCCTCCTCCAAAAAGAACCCATTTCATTTTCCCGCACTTTCTCA
CCCCAAACGATTCTCGCCCGGAAAATGAGGTGGGAAAGGGTCCGACAACTACAGCAACAA
GTGGGTCTCGGTGAGTCTTCTTCCGGGCCCGGTAAGAGGTGGGGTCACACTTGTAACGCC
ATTAAAGGAGGTAGCTTTCTCTATGTTTTCGGTGGCTATGGCAGAGATAATTGCCAAACC
AATCAAGTCCATGTTTTCGACGCTGGTTAGTTCTCTCTTTCCACATCGTCTCTGTTCTCA
AAAAATGGCGGATTTGGGTTGATTCCCATTTCGGATTTCGATTCTAGACAATCATCTAAG
TTTCCTACCTTTGTTCATCCATATNGCATGTCTA

Last Updated on 06.12.2018 16:11